+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro)



Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro)



Plant Cell Reports 30(11): 2045-2057



Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A normalized cDNA library was constructed from young leaves of Ma bamboo and 9,574 high-quality ESTs were generated, from which 5,317 unigenes including 1,502 contigs and 3,815 singletons were assembled. The unigenes were assigned into different gene ontology (GO) categories and summarized into 13 broad biologically functional groups according to similar functional characteristics or cellular roles by BLAST search against public databases. Eight hundred and ninety-one unigenes were assigned by KO identifiers and mapped to six KEGG biochemical pathways. The transcripts involved in biosynthesis of secondary metabolites such as cytochrome 450, flavonol synthase/flavanone 3-hydroxylase, and dihydroflavonol-4-reductase were well represented by 14 unigenes in the unigene set. The candidate genes involved in phytohormone metabolism, signal transduction and encoding cell wall-associated receptor kinases were also identified. Sixty-seven unigenes related to plant resistance (R) genes, including RPP genes, RGAs and RDL/RF genes, were discovered. These results will provide genome-wide knowledge about the molecular physiology of Ma bamboo young leaves and tools for advanced studies of molecular mechanism underlying leaf growth and development.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036138448

Download citation: RISBibTeXText

PMID: 21713530

DOI: 10.1007/s00299-011-1112-0


Related references

Generation of expressed sequence tags from a normalized porcine skeletal muscle cDNA library. Animal Biotechnology 13(2): 211-222, 2003

Expressed sequence tags for the chicken genome from a normalized 10-day-old White Leghorn whole embryo cDNA library: 1. DNA sequence characterization and linkage analysis. Journal of Heredity 92(1): 1-8, 2001

Transcriptome sequencing and de novo analysis for Ma bamboo (Dendrocalamus latiflorus Munro) using the Illumina platform. Plos One 7(10): E46766, 2013

Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug Phenacoccus solenopsis. Insect Science 22(6): 761-767, 2016

A large scale analysis of cDNA in Arabidopsis thaliana: generation of 12,028 non-redundant expressed sequence tags from normalized and size-selected cDNA libraries. Dna Research 7(3): 175-180, 2000

Propagation of Ma Chu bamboo (Dendrocalamus latiflorus) and Lu Chu bamboo (Bambusa oIdhamii Munro.) by culm cutting. Proceedings of the 45th Kasetsart University Annual Conference, Bangkok, Thailand, 30 January 2 February 2007 Subject: Plants: 502-509, 2007

Expressed sequence tags for the chicken genome from a normalized, ten-day-old white leghorn whole embryo cDNA library. 2. Comparative DNA sequence analysis of guinea fowl, quail, and turkey genomes. Poultry Science 80(9): 1263-1272, 2001

Generation and analysis of expressed sequence tags from a cDNA library of Tamarix androssowii. Plant Science 170(1): 28-36, 2006

Generation and analysis of expressed sequence tags from a cDNA library of Moniezia expansa. Molecular and Biochemical Parasitology 164(1): 80-85, 2009

Expressed sequence tags for the chicken genome from a normalized 10-day-old white leghorn whole-embryo cDNA library. 3. DNA sequence analysis of genetic variation in commercial chicken populations. Genome 45(2): 261-267, 2002

Germination and cultivation of seedlings of giant bamboo (Dendrocalamus latiflorus Munro). Research Note Taiwan Forestry Research Institute (2): 14, 1985

Occurrence and flowering of a bamboo Dendrocalamus latiflorus Munro, in Nagaland, India. Indian Forester 132(3): 358-364, 2006

Generation and analysis of expressed sequence tags from a cDNA library of the fruiting body of Ganoderma lucidum. Chinese Medicine 5: 9, 2010

Generation and analysis of expressed sequence tags from a NaHCO3-treated Limonium bicolor cDNA library. Plant Physiology and Biochemistry 46(11): 977-986, 2008