+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Two new effective semiselective crystal violet pectate media for isolation of Pectobacterium and Dickeya

Two new effective semiselective crystal violet pectate media for isolation of Pectobacterium and Dickeya

Plant Pathology 61(2): 339-345

Pectolytic bacteria, including Pectobacterium spp. and Dickeya spp., are best isolated on crystal violet pectate (CVP), a semiselective medium containing pectin. The source of pectin is essential, because pectolytic bacteria are not able to degrade all of them. The aims of this study were to identify a new pectin source and to perfect formulations of semiselective CVP media to isolate the pectolytic bacteria Pectobacterium spp. and Dickeya spp. from different environmental compartments (plants, soil and water). The AG366 pectin, selected after screening six different formulations, was incorporated into single-layer (SL-CVPAG366) and double-layer (DL-CVPAG366) CVP media. Both media were compared with those based on Bulmer, Sigma-Aldrich and Slendid-Burger pectins, using 39 Pectobacterium and Dickeya strains. All strains formed deep cavities on AG366-CVPs, whereas nine did not produce cavities on Bulmer or Sigma-Aldrich media. Recovery rates were similar on DL-CVPAG366, Sigma-Aldrich and Bulmer CVPs for a given taxon, and did not differ significantly between SL- and DL-CVPAG366. Pectolytic bacteria were successfully isolated on both media from field samples of diseased potatoes, carrots, tobacco, onions, radishes and ornamentals. AG366 is thus a high-performance pectin source for the elaboration of CVP media suitable to isolate Dickeya and Pectobacterium. It is also efficient for enrichment purposes in liquid medium. The validation of AG366 as an improved source of pectin to recover the polyphagous Pectobacterium and Dickeya in different environmental compartments is essential given the current worldwide emergence and recrudescence of these bacteria.

(PDF emailed within 0-6 h: $19.90)

Accession: 036152203

Download citation: RISBibTeXText

DOI: 10.1111/j.1365-3059.2011.02508.x

Related references

Two modified crystal violet pectate (CVP) media for the detection, isolation and enumeration of soft rot erwinias. Potato Research 34(1): 79-85, 1991

Comparative efficiency of crystal violet pectate and sodium poly pectate media on the recovery of pectolytic erwinia from soil following enrichment. Phytopathology 71(9): 1008, 1981

Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. International Journal of Systematic and Evolutionary Microbiology 55(Pt 4): 1415-1427, 2005

A modified crystal violet pectate (CVP) medium for detection and isolation of soft rot Erwinia spp. from plant materials. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 111(5): 506-515, 2004

Modified crystal violet pectate medium (CVP) based on a new polypectate source (Slendid) for the detection and isolation of soft rot erwinias. Potato Research 44(3): 265-270, 2001

Virulence of Pectobacterium carotovorum subsp brasiliense on potato compared with that of other Pectobacterium and Dickeya species under climatic conditions prevailing in the Netherlands. Plant Pathology 66(4): 571-583, 2017

Analysis of incurred crystal violet in Atlantic salmon (Salmo salar L.): comparison between the analysis of crystal violet as an individual parent and leucocrystal violet and as total crystal violet after oxidation with 2,3-dichloro-5,6-dicyanobenzoquinone. Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment 29(1): 66-72, 2012

Application of zinc chloride precipitation method for rapid isolation and concentration of infectious Pectobacterium spp. and Dickeya spp. lytic bacteriophages from surface water and plant and soil extracts. Folia Microbiologica 61(1): 29-33, 2016

An improved formulation and method of preparation of crystal violet pectate medium for detection of pectolytic Erwinia. Letters in Applied Microbiology 10(4): 171-173, 1990

Research on Dickeya and Pectobacterium. Phytopathology 108(10), 2018

Semiselective media for isolation of Phaeomoniella chlamydospora from vine and soil samples. Phytopathologia Mediterranea 46(1): 108-109, 2007

Semiselective media for the isolation of elsinoe fawcettii from citrus scab pustules. Plant Disease 70(3): 204-206, 1986

Immunomagnetic separation of Erwinia carotovora subsp. atroseptica from potato peel extracts to improve detection sensitivity on a crystal violet pectate medium or by PCR. Journal of Applied Bacteriology 80(5): 487-495, 1996

Tween media for semiselective isolation of Xanthomonas campestris pv. vesicatoria from soil and plant material. Plant Disease 70(9): 887-891, 1986

Why Genomics Research on Pectobacterium and Dickeya Makes a Difference. American Journal of Potato Research 92(2): 218-222, 2015