+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

A protocol for enumeration of aquatic viruses by epifluorescence microscopy using AnodiscTM 13 membranes

, : A protocol for enumeration of aquatic viruses by epifluorescence microscopy using AnodiscTM 13 membranes.

Epifluorescence microscopy is a common method used to enumerate virus-like particles (VLP) from environmental samples and relies on the use of filter membranes with pore sizes <.2 um; the most commonly used protocols employ 25 mm AnodiscTM membranes with a built-in support ring. Other filters with small pore sizes exist, including the 13 mm AnodiscTM membranes without a support ring. However, the use of these membranes for viral enumeration has not been previously reported. Here we describe a modified protocol for 13 mm Anodisc membranes that uses a custom filter holder that can be readily constructed in individual investigators' laboratories from commercially available Swinnex(R) filter holders. We compared VLP concentrations obtained from phage lysates and seawater samples using both Anodisc membranes, as well as NucleporeTM small pore-size membranes (.15 or .3 um). The 13 mm Anodisc membranes gave comparable estimates of VLP abundance to those obtained with the 25 mm Anodisc membranes when similar staining methods were employed. Both Nuclepore membranes typically gave an order of magnitude lower VLP abundance values for environmental samples. The 13 mm Anodisc membranes are less costly and require smaller sample volumes than their 25 mm counterpart making them ideal for large-scale studies and sample replication. This method increases the options of reliable approaches available for quantifying VLP from environmental samples.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 036161387

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Harvey, T. L.; Brethour, J. R., 1970: Horn fly control with dichlorvos-impregnated strips. Ear tags, neck collars, and strips and blocks (suspended from neck chains) were cut from resin strips containing 20% dichlorvos and tested against Siphona (Haemalobia) irritons on cattle at pasture. Protection was satisfactory for periods ranging...

Gowda, K. T. K.; Krishnamurthy, K., 1977: Response of sesamum varieties to spacings and fertilizer levels. In trials with 3 sesame cv. given 20-60 kg N, 20 kg P2O5 and 20-40 kg K2O/ha, av. seed yields with between-plant spacings of 10, 20 or 30 cm in rows 30 cm apart were 823.4, 738.8 and 627.7 kg/ha, resp. Yields with N + P2O5 + K2O at respective rate...

O'Leary, Maureen A., 2010: An anatomical and phylogenetic study of the osteology of the petrosal of extant and extinct artiodactylans Mammalia and relatives. I describe and figure petrosal bones for a sample of 35 (12 extinct, 23 extant) artiodactylans, perissodactylans, mesonychids, and archaic ungulates. Detailed herein are the cladistic characters of the petrosal used in the recent combined analysis...

Bennett F.A., 1970: Variable density yield tables for managed stands of natural slash pine g. U S Forest Service Research Note SE 141: 1-7

Matig, O. E., 1989: Attempts to rehabilitate sterile soils - forest plantations in north Cameroon. In the northernmost province of Cameroon, 25 to 30% of the land is composed of sterile (local name: harde) or degraded soils; these have been assigned for reforestation work planned for the Cameroonian part of the Sahel where forestry research is...

Nuorteva, P., 1966: The occurrence of Phormia terrae-novae R.-D. (Dipt., Calliphoridae) and other blowflies in the archipelago of the subarctic lake Inarinjarvi. Suomen Hyonteistieteellinen Aikakauskirja, 32: 240-251

Vanzolini, PE., 1972: Repteis e anfibios ameacados de extincdo no Brasil. Unknown

Holdsworth, M., 1971: Breeding biology of buzzards at Sedburgh during 1937-67. British Birds, 64: 412-420

Lourteig, A., 1988: Nomenclatura plantarum americanarum vii. leguminosae. Over the centuries names which do not correspond to the International Code of Botanical Nomenclature have been used for various Leguminosae. That situation was corrected for species for Canavalia, Clitoria, Lonchocarpus and Senna from the Antilles...

Bernhauer W., 1957: Ornithological observations on the adriatic coast. Larus (English Translation) 9-10: (1973)