+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Interleukin (IL)11 mediates protein secretion and modification in human extravillous trophoblasts

Interleukin (IL)11 mediates protein secretion and modification in human extravillous trophoblasts

Human Reproduction 26(10): 2841-2849

Human trophoblast invasion and differentiation are essential for a successful pregnancy outcome. Dysregulation of these processes can lead to placental pathologies such as pre-eclampsia. The molecular mechanisms; however, are poorly understood. Interleukin (IL)11--a cytokine that regulates endometrial epithelial cell adhesion, trophoblast motility and invasion during implantation--may be involved in some of these processes. The effect of IL11 on protein expression was investigated in trophoblastic HTR8/SVneo cells and primary extravillous trophoblasts (EVTs) purified from first- trimester placentas. Two-dimension (2D)-differential in-gel electrophoresis analyses revealed that 731 spots were significantly differentially regulated by IL11 in HTR8/SVneo cells: seven spots were analyzed by liquid chromatography-tandem mass spectrometry and 14 unique proteins identified. Protein disulfide isomerase family A, member 3 (PDIA3; endoplasmic reticulum p57) and glucose-regulated protein 78 (GRP78) were further validated to be regulated by IL11 in HTR8/SVneo and primary EVT. One dimension western blot analysis confirmed that PDIA3 was down-regulated in EVT. 2D western blot analysis revealed that GRP78 was post-translationally modified following IL11 treatment. Moreover, IL11 stimulated the secretion of GRP78 in EVT. Data suggest that IL11, possibly via signal transducers and activators of transcription 3 signaling pathway, regulates PDIA3 protein expression and modification/secretion of GRP78. This is the first study to identify PDIA3 and GRP78 as IL11 targets in invasive trophoblasts and identifies a possible mechanism by which IL11 regulates trophoblast function.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036203542

Download citation: RISBibTeXText

PMID: 21840908

DOI: 10.1093/humrep/der259

Related references

Expression of Epstein-Barr virus-induced gene 3, an interleukin-12 p40-related molecule, throughout human pregnancy: involvement of syncytiotrophoblasts and extravillous trophoblasts. American Journal of Pathology 159(5): 1763-1776, 2001

Human extravillous trophoblasts express laeverin, a novel protein that belongs to membrane-bound gluzincin metallopeptidases. Biochemical and Biophysical Research Communications 313(4): 962-968, 2004

Isolation and molecular studies of human HLA-G+ extravillous trophoblasts (EVT). Journal of Reproductive Immunology 86(1): 32-0, 2010

Human extravillous trophoblasts bind but do not internalize antiphospholipid antibodies. Placenta 42: 9, 2016

Lack of human leukocyte antigen-G expression in extravillous trophoblasts is associated with pre-eclampsia. Molecular Human Reproduction 6(1): 88-95, 2000

Ikaros is expressed in human extravillous trophoblasts and involved in their migration and invasion. Molecular Human Reproduction 11(11): 825-831, 2005

Characterization of human villous and extravillous trophoblasts isolated from first trimester placenta. Laboratory Investigation 81(9): 1199-1211, 2001

Endostatin suppresses IGF-II-mediated signaling and invasion of human extravillous trophoblasts. Endocrinology 152(11): 4431-4442, 2011

Protein Kinase C Mediates Lysophosphatidic Acid-induced NF-kB Activation and Interleukin-8 Secretion in Human Bronchial Epithelial Cells. Journal of Biological Chemistry 279(39 S 24 2004): 085-94, 2004

Tetrabromobisphenol A activates inflammatory pathways in human first trimester extravillous trophoblasts in vitro. Reproductive Toxicology 50: 154-162, 2014

Calreticulin Is Involved in Invasion of Human Extravillous Trophoblasts Through Functional Regulation of Integrin β1. Endocrinology 158(11): 3874-3889, 2017

Insulin Exhibits an Antiproliferative and Hypertrophic Effect in First Trimester Human Extravillous Trophoblasts. Reproductive Sciences 24(4): 582-594, 2017

Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes. Proceedings of the National Academy of Sciences of the United States of America 112(23): 7219-7224, 2015

Human migrating extravillous trophoblasts express a cell surface peptidase, carboxypeptidase-M. Molecular Human Reproduction 9(12): 799-806, 2003

Expression of GALNT2 in human extravillous trophoblasts and its suppressive role in trophoblast invasion. Placenta 33(12): 1005-1011, 2012