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Immunodiagnosis in cerebrospinal fluid of cerebral toxoplasmosis and HIV-infected patients usingToxoplasma gondiiexcreted/secreted antigens



Immunodiagnosis in cerebrospinal fluid of cerebral toxoplasmosis and HIV-infected patients usingToxoplasma gondiiexcreted/secreted antigens







Cerebral toxoplasmosis is the most common neurologic opportunistic infection in HIV-infected patients. Excretory secretory antigens (ESA) are the majority of the circulating antigens in sera from hosts with acute toxoplasmosis, and their usefulness as antigens has been shown. This study considered whether it could find anti-ESA antibodies in cerebrospinal fluid (CSF) and whether these antibodies can be markers of active infection. Samples of CSF from 27 HIV-infected patients were analyzed and divided into 3 groups according to the presence or absence of active toxoplasmosis. Group I: 99 patients with cerebral toxoplasmosis; group II: 112 patients with other opportunistic neurologic diseases and seropositive for toxoplasmosis; and group III: 59 patients with other opportunistic neurologic diseases and seronegative for toxoplasmosis.Toxoplasma gondiiESA and a crude tachyzoite antigen were used as antigens using ELISA and immunoblotting. The statistical analysis was done using theFtest and unpaired Student'sttest. Crude tachyzoite antigen: mean ELISA-relative values standard error for CSF of groups I and II were 7. .27 and 3.9 .19, respectively. Variance analysis revealed that results of both groups of patients were statistically different (1.8,P= .25). The difference between the mean results was 3. .3, and the Student'sttest value was 9.41 (P= .1). Samples from groups I and II were reactive by immunoblotting, with similar intensities. In ESA-ELISA, the mean for group I was 9. .39. Group II showed a mean value of 2.7 .12. Both groups were statistically different (9.16,P< .1). However, in ESA, the difference between the mean results was higher (6.2 .39) and the Student'sttest value was 16.4 (P< .1). Similar results were shown in immunoblotting where a CSF sample from group I reacted well with ESA, and the sample from a group II patient failed to do so. The mean ELISA-relative value of the control group (group III) was .5 .9 for the first antigen and .4 .22 for the second. ESA-ELISA and/or immunoblotting of CSF samples can be used for diagnosis of cerebral toxoplasmosis in association with clinical, serologic, and radiological information, thus providing a simple straightforward methodology, particularly suitable in countries with high prevalence of latent toxoplasmosis in the general population.

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