+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Autoregulatory circuit of human rpL3 expression requires hnRNP H1, NPM and KHSRP

Autoregulatory circuit of human rpL3 expression requires hnRNP H1, NPM and KHSRP

Nucleic Acids Research 39(17): 7576-7585

Alternative pre-mRNA splicing (AS) is a major mechanism that allows proteomic variability in eukaryotic cells. However, many AS events result in mRNAs containing a premature termination codon, which are degraded by nonsense-mediated mRNA decay (NMD) pathway. We have previously demonstrated that human rpL3 autoregulates its expression through the association of AS with NMD. In fact, overexpression of rpL3 promotes downregulation of canonical splicing and upregulation of alternative splicing that produces an NMD-targeted mRNA isoform. The result of these events is a decreased production of rpL3. We have also identified heterogeneous nuclear ribonucleoprotein (hnRNP) H1 as a splicing factor involved in the regulation of rpL3 alternative splicing and identified its regulatory cis-elements within intron 3 transcript. Here, we report that NPM and KHSRP are two newly identified proteins involved in the regulation of rpL3 gene expression via AS-NMD. We demonstrate that hnRNP H1, KHSRP and NPM can be found associated, and present also in ribonucleoproteins (RNPs) including rpL3 and intron 3 RNA in vivo, and describe protein-protein and RNA-protein interactions. Moreover, our data provide an insight on the crucial role of hnRNP H1 in the regulation of the alternative splicing of the rpL3 gene.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036207656

Download citation: RISBibTeXText

PMID: 21705779

DOI: 10.1093/nar/gkr461

Related references

hnRNP H1 and intronic G runs in the splicing control of the human rpL3 gene. Biochimica et Biophysica Acta 1799(5-6): 419-428, 2010

Antizyme expression: a subversion of triplet decoding, which is remarkably conserved by evolution, is a sensor for an autoregulatory circuit. Nucleic Acids Research 28(17): 3185-3196, 2000

NOTCH3 expression is induced in mural cells through an autoregulatory loop that requires endothelial-expressed JAGGED1. Circulation Research 104(4): 466-475, 2009

DHX34 and NBAS form part of an autoregulatory NMD circuit that regulates endogenous RNA targets in human cells, zebrafish and Caenorhabditis elegans. Nucleic Acids Research 41(17): 8319-8331, 2013

Expression profile and interactions of hnRNP A3 within hnRNP/mRNP complexes in mammals. Archives of Biochemistry and Biophysics 523(2): 151-160, 2012

Effects of hnRNP B1-specific siRNA on the expression of hnRNP B1 in lung cancer tissues in vivo. Sichuan Da Xue Xue Bao. Yi Xue Ban 39(5): 808-811, 2008

BRCA1 modulates the expression of hnRNPA2B1 and KHSRP. Cell Cycle 9(23): 4666-4673, 2011

HnRNP E2 is downregulated in human oral cancer cells and the overexpression of hnRNP E2 induces apoptosis. Molecular Carcinogenesis 46(3): 198-207, 2007

Nucleo-cytoplasmic distribution of human hnRNP proteins: a search for the targeting domains in hnRNP A1. Journal of Cell Science 108: 545-555, 1995

Lnteroceptive-interomotor autoregulatory circuit of the smooth muscles. Acta Univ Palackianae Olomucensis 27: 115-121, 1961

Proprioceptive-exteromotor autoregulatory circuit of the striated muscles. Acta Univ Palackianae Olomucensis 27: 105-114, 1961

Camptothecin (CPT) directly binds to human heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and inhibits the hnRNP A1/topoisomerase I interaction. Bioorganic and Medicinal Chemistry 19(24): 7690-7697, 2012

Organization of the human gene encoding heterogeneous nuclear ribonucleoprotein type I (hnRNP I) and characterization of hnRNP I related pseudogene. Gene (Amsterdam) 255(2): 267-272, 2000

An autoregulatory circuit affecting peptide signaling in Bacillus subtilis. Journal of Bacteriology 181(17): 5193-5200, 1999