Postharvest biocontrol of Monilinia laxa, Monilinia fructicola and Monilinia fructigena on stone fruit by two Aureobasidium pullulans strains

Marta Mari; Camilla Martini; Michela Guidarelli; Fiorella Neri

Biological Control 60(2): 132-140

2012


ISSN/ISBN: 1049-9644
DOI: 10.1016/j.biocontrol.2011.10.013
Accession: 036244654

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Abstract
The antagonistic effects of yeasts,L1 and L8, isolated from carposphere of Redhaven peaches were tested for the first time in the same experiment against threeMoniliniaspecies (M. laxa, M. fructicolaandM. fructigena) inin-vitroandin-vivotrials. The two antagonists were selected after preliminary assays for their ability to reduce brown rot in peaches and nectarines, and both were identified by molecular and morphological tools asAureobasidium pullulans. Inin-vivotrials, neither the autoclaved cells, nor the sterile culture filtrates of either antagonist showed any significant reduction of rot incidence produced by inocula of the threeMoniliniaspecies, while the washed cells of L1 and L8 completely inhibitedM. laxaand M.fructicolarots and reducedM. fructigenainfections by 7% and 9%, respectively. In other trials, nectarines treated with antagonist cells and inoculated with the pathogens were stored at C for 21 days, plus 7 days at 2 C. The low temperature reduced brown rot development, since all fruit were free from disease symptoms on removal from cold storage. However after 7 d at 2 C, untreated fruit were rotted over 45% depending on theMoniliniaspecies but the antagonists completely inhibitedM. laxaandM. fructicola, whileM. fructigenainfections were reduced by89.8% and 91.2% by L1 and L8, respectively. For both strains,1cfu mlwas the most active concentration, althoughL1 showed good activity at a concentration of 1CFU ml.IsolateL8 at the concentration of 1CFU mlwas ineffective againstM. fructicolaandM. fructigena, showing no difference between treated fruit and the control, excepting the case of nectarines inoculated withM. laxa, where L8 at the concentration of1cfu mlreducedthe brown rot infections with respect to the control. The increase in population density ofA. pullulansstrains L1 and L8 in the wounds of nectarines stored at or 2 C was low but sufficient to control brown rot. In conclusion, the present preliminary study identified two antagonistic strains ofA. pullulansas active ingredients for the development of biofungicides for postharvest application against threeMoniliniaspecies that are responsible for high economic losses in stone fruit crops.The antagonistic effect of two yeasts was tested in the same experiment against M. laxa, M. fructicolaand M. fructigena. The two antagonists were identified by molecular and morphological tools as Aureobasidium pullulans. The washed cells of antagonists completely inhibited M. laxaand fructicolarots and reduced M. fructigenainfections. Low temperature did not influence antagonist efficacy, M. laxaand M. fructicolawere completely inhibited by antagonists.