+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris



High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris



Biotechnology Letters 34(4): 689-694



The sequence of an endo-chitosanase gene (CSN) from Aspergillus fumigatus was optimized based on the preferred codons of Pichia pastoris and synthesized in vitro through overlapping PCR (CSN-P). The gene was cloned into a yeast expression vector, pHBM905A, and secretorily expressed in Pichia pastoris GS115. The yield of CSN-P reached ~3 mg/ml with a high-density fermentation in a 14 l fermenter and the enzyme activity was ~25,000 U/ml. The enzyme had half-lives of 2.5 h at 80°C, 1 h at 90°C and 32 min at 100°C. It retained 70% activity after incubation with 10 M urea at room temperature for 30 min. This enzyme was used for a large-scale preparation of oligosaccharides: 3 g enzyme converted 200 kg chitosan into oligosaccharides in 24 h at 60°C.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036289557

Download citation: RISBibTeXText

PMID: 22160328

DOI: 10.1007/s10529-011-0816-0


Related references

Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33. Microbial Cell Factories 11: 25, 2012

Heterologous expression of Aspergillus fumigatus chitosanase cDNA in Pichia pastoris. Mycobiology 29(4): 230-231, 2001

High-level expression and characterization of a thermostable xylanase mutant from Trichoderma reesei in Pichia pastoris. Protein Expression and Purification 108: 90-96, 2015

Cloning, expression and characterization of endo-beta-1,4-mannanase from Aspergillus fumigatus in Aspergillus sojae and Pichia pastoris. Biotechnology Progress 25(1): 271-276, 2009

Expression and characterization of Aspergillus thermostable phytases in Pichia pastoris. Fems Microbiology Letters 290(1): 18-24, 2008

Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein. Applied Microbiology and Biotechnology 101(14): 5653-5666, 2017

High-level expression and characterization of a thermophilic β-mannanase from Aspergillus niger in Pichia pastoris. Biotechnology Letters 37(9): 1853-1859, 2015

High-level expression and characterization of a stereoselective lipase from Aspergillus oryzae in Pichia pastoris. Protein Expression and Purification 155: 1-7, 2019

Expression of the Aspergillus fumigatus phytase gene in Pichia pastoris and characterization of the recombinant enzyme. Biochemical and Biophysical Research Communications 268(2): 373-378, 2000

A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis. International Journal of Molecular Sciences 20(9):, 2019

High-level expression, purification and characterization of recombinant Aspergillus oryzae alkaline protease in Pichia pastoris. Protein Expression and Purification 58(2): 301-308, 2008

High-level expression of Aspergillus niger lipase in Pichia pastoris: Characterization and gastric digestion in vitro. Food Chemistry 274: 305-313, 2019

Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris. Biomed Research International 2016: 5962028, 2016

High-level expression, purification, and enzymatic characterization of a recombinant Aspergillus sojae alkaline protease in Pichia pastoris. Protein Expression and Purification 148: 24-29, 2018

High-level expression, purification, and enzymatic characterization of a recombinant Aspergillus sojae alkaline protease in Pichia pastoris. Protein Expression and Purification 148: 24-29, 2018