EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

The use of Pseudomonas fluorescens P13 to control sclerotinia stem rot (Sclerotinia sclerotiorum) of oilseed rape


The use of Pseudomonas fluorescens P13 to control sclerotinia stem rot (Sclerotinia sclerotiorum) of oilseed rape



Journal of Microbiology 49(6): 884-889



ISSN/ISBN: 1225-8873

PMID: 22203550

DOI: 10.1007/s12275-011-1261-4

Sclerotinia stem rot (SSR) caused by the fungus Sclerotinia sclerotiorum has been an increasing threat to oilseed rape (Brassica napus L.) cultivation. Efficient and environment-friendly treatments are much needed. Here we focus on microbial control. The Pseudomonas fluorescens P13 that was isolated from oilseed rape cultivation soil, proved to be a useful biocontrol strain for application. Morphology, physiological and biochemical tests and 16S rDNA analysis demonstrated that it was P. fluorescens P13 and that it had a broad antagonistic spectrum, significantly lessening the mycelial growth of S. sclerotiorum by 84.4% and suppressing sclerotial formation by 95-100%. Scanning electron microscopy studies attested that P13 deformed S. sclerotiorum mycelia when they were cultured together. P13 did not produce chitinase but did produce hydrogen cyanide (HCN) which was likely one of the antagonistic mechanisms. The density of P13 remained at a high level (≥10(6) CFU/ml) during 5 weeks in the rhizosphere soil and roots. P13 reduced SSR severity at least by 59% in field studies and also promoted seedling growth (p<0.05) at the seedling stage. From these data, our work provided evidence that P13 could be a good alternative biological resource for biocontrol of S. sclerotiorum.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036301177

Download citation: RISBibTeXText

Related references

Exploring fungal DNA virus to control stem rot of oilseed rape caused by Sclerotinia sclerotiorum. Phytopathology 107(12): 147-147, 2017

Biocontrol of reinfection of oilseed rape stem rot caused by Sclerotinia sclerotiorum by Coniothyrium minitans and its survival on leaf of oilseed rape (Brassica napus). Acta Phytopathologica Sinica 30(1): 60-65, 2000

Components of a Rice-Oilseed Rape Production System Augmented with Trichoderma sp. Tri-1 Control Sclerotinia sclerotiorum on Oilseed Rape. Phytopathology 105(10): 1325-1333, 2015

Beeinflussung der Sklerotienkeimung von Sclerotinia sclerotiorum, dem Erreger der Weissstengeligkeit des Rapses, durch Umwandlungsprodukte von Kalkstickstoff - L'influence des produits de transformation du cyanamide de chaux sur la germination des sclérotes de Sclerotinia sclerotiorum, l'agent causal du chancre du colza - The effect of transformation products of calcium cyanamide on the sclerotial germination of Sclerotinia sclerotinia sclerotiorum, the fungus causing rape canker. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz (1970) 92(5): 449-454, 1985

First Report of Oilseed Rape Stem Rot Caused by Sclerotinia sclerotiorum in Greece. Plant Disease 92(10): 1473, 2008

Evaluation of non-injury inoculation technique for assessing Sclerotinia stem rot (Sclerotinia sclerotiorum) in oilseed Brassica. Journal of Microbiological Methods 175: 105983, 2020

Valuable New Resistances Ensure Improved Management of Sclerotinia Stem Rot (Sclerotinia sclerotiorum) in Horticultural and Oilseed Brassica Species. Journal of Phytopathology 164(5): 291-299, 2016

Prothioconazole for control of Sclerotinia sclerotiorum in oilseed rape/canola. Pflanzenschutz-Nachrichten Bayer 57(2): 283-293, 2004

The control of Sclerotinia sclerotiorum on oilseed rape: what future for carbendazim?. Phytoma 529: 31-33, 2000

Control of Sclerotinia sclerotiorum (Lib.) de Bary on oilseed rape in France. Aspects of applied biology: ) 355-360, 1984

Effects of the Novel Fungicide Benzothiostrobin on Sclerotinia sclerotiorum in the Laboratory and on Sclerotinia Stem Rot in Rape Fields. Plant Disease 99(7): 969-975, 2015

Etudes du pouvoir pathogène de huit isolats de Sclerotinia appartenant aux espèces Sclerotinia sclerotiorum, Sclerotinia minor et Sclerotinia trifoliorum sur tournesol - Study of the pathogenic power of eight Sclerotinia isolates belonging to the species S. sclerotiorum, S. minor and S. trifoliorumon sunflower. Informations Techniques - C.E.T.I.O.M (103): 21-27, 1988

Evaluation of fungicides for control of (Sclerotinia sclerotiorum) on winter oilseed rape. Tests of Agrochemicals and Cultivars (16): 18-19, 1995

Bacillus megaterium A6 suppresses Sclerotinia sclerotiorum on oilseed rape in the field and promotes oilseed rape growth. Crop Protection 52: 151-158, 2013

Control of Sclerotinia sclerotiorum infection in oilseed rape with strobilurin fungicide SYP-7017. Canadian Journal of Plant Pathology 36(3): 354-359, 2014