+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Angiotensin-(1-7) attenuates high glucose-induced proximal tubular epithelial-to-mesenchymal transition via inhibiting ERK1/2 and p38 phosphorylation



Angiotensin-(1-7) attenuates high glucose-induced proximal tubular epithelial-to-mesenchymal transition via inhibiting ERK1/2 and p38 phosphorylation



Life Sciences 90(11-12): 454-462



The kidney is an important target for both Angiotensin II and angiotensin-(1-7) [Ang-(1-7)] in the renin-angiotensin system. However, the renal function of Ang-(1-7) remains unclear. This study is aimed at investigating the effect of Ang-(1-7) on high glucose-induced epithelial to mesenchymal transition (EMT) in cultured renal epithelial cells. Cultured renal epithelial (NRK-52E) cell line was used in the experiment. Fluorescence immunocytochemistry was performed to observe α-smooth muscle actin (α-SMA). Real-time PCR and Western blot were used to determine mRNA and protein levels. Enzyme-linked immunosorbent assay was used to measure the concentration of transforming growth factor-β1 (TGF-β1) in the culture media. High glucose-induced decreased in both angiotensin-converting enzyme-related carboxypeptidase (ACE2) and Mas mRNA levels. Meanwhile, high glucose induced increases in α-SMA and vimentin, decreases in E-cadherin, elevations in TGF-β1 and fibronectin secretions. Ang-(1-7) partially reversed high glucose-induced changes in α-SMA, vimentin, E-cadherin, TGF-β1 and fibronectin. High glucose stimulated ERK, p38 and JNK phosphorylation and Ang-(1-7) reversed the changes in ERK and p38 but not JNK phosphorylation. Inhibition and insufficiency in ACE2-Ang-(1-7)-Mas axis under high glucose condition participate EMT. Supplementation of Ang-(1-7) attenuates high glucose-induced EMT. ERK and p38 intracellular signaling pathways, not JNK, mediate the effect of Ang-(1-7) on EMT.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036315767

Download citation: RISBibTeXText

PMID: 22285598

DOI: 10.1016/j.lfs.2011.12.015


Related references

Angiotensin AT1 receptor activation mediates high glucose-induced epithelial-mesenchymal transition in renal proximal tubular cells. Clinical and Experimental Pharmacology and Physiology 37(9): E152-E157, 2011

Silencing of angiotensin II type-1 receptor inhibits high glucose-induced epithelial-mesenchymal transition in human renal proximal tubular epithelial cells via inactivation of mTOR/p70S6K signaling pathway. Biochemical and Biophysical Research Communications 469(2): 183-188, 2016

Astragaloside IV attenuates glycated albumin-induced epithelial-to-mesenchymal transition by inhibiting oxidative stress in renal proximal tubular cells. Cell Stress and Chaperones 19(1): 105-114, 2014

AEG-1 participates in high glucose-induced activation of Rho kinase and epithelial-mesenchymal transition in proximal tubular epithelial cells. Asian Pacific Journal of Tropical Medicine 8(12): 1076-1078, 2015

Procyanidin B2 inhibits high glucose‑induced epithelial‑mesenchymal transition in HK‑2 human renal proximal tubular epithelial cells. Molecular Medicine Reports 12(6): 8148-8154, 2016

Resveratrol prevents high glucose-induced epithelial-mesenchymal transition in renal tubular epithelial cells by inhibiting NADPH oxidase/ROS/ERK pathway. Molecular and Cellular Endocrinology 402: 13-20, 2015

Modulatory effect of betanin on high glucose induced epithelial to mesenchymal transition in renal proximal tubular cells. Biomedicine and PharmacoTherapy 89: 18-28, 2017

Lefty A attenuates the TGF-beta1-induced epithelial to mesenchymal transition of human renal proximal epithelial tubular cells. Molecular and Cellular Biochemistry 339(1-2): 263-270, 2010

Paricalcitol attenuates 4-hydroxy-2-hexenal-induced inflammation and epithelial-mesenchymal transition in human renal proximal tubular epithelial cells. Plos One 8(5): E63186, 2013

Hirsutella sinensis Attenuates Aristolochic Acid-Induced Renal Tubular Epithelial-Mesenchymal Transition by Inhibiting TGF-β1 and Snail Expression. Plos One 11(2): E0149242, 2016

GSTA3 Attenuates Renal Interstitial Fibrosis by Inhibiting TGF-Beta-Induced Tubular Epithelial-Mesenchymal Transition and Fibronectin Expression. Plos One 11(9): E0160855, 2017

High glucose induce epithelial-mesenchymal transition in renal proximal tubular cells through PERK-eIF2α pathway. Chinese Medical Journal 2019, 2019

Nudel involvement in the high glucose-induced epithelial-mesenchymal transition of tubular epithelial cells. American Journal of Physiology. Renal Physiology 2018, 2018

CD36 is involved in high glucose-induced epithelial to mesenchymal transition in renal tubular epithelial cells. Biochemical and Biophysical Research Communications 468(1-2): 281-286, 2016

Effect of zinc on high glucose-induced epithelial-to-mesenchymal transition in renal tubular epithelial cells. International Journal of Molecular Medicine 35(6): 1747-1754, 2016