+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isoquinoline alkaloids from Tinospora cordifolia inhibit rat lens aldose reductase

Isoquinoline alkaloids from Tinospora cordifolia inhibit rat lens aldose reductase

PhytoTherapy Research 26(9): 1342-1347

The inhibitory activity of Tinospora cordifolia stem-derived alkaloids was evaluated against lens aldose reductase (AR) isolated from male Wistar rats. Anticataract potential of the alkaloids of T. cordifolia was evaluated in vitro in rat lenses, considering the activity of normal rat lenses as 100%. The biologically active constituents of T. cordifolia extract were characterized as the isoquinoline alkaloids, jatrorrhizine, palmatine and magnoflorine, by spectral analysis. The inhibitory effects varied with all chemicals and concentrations used. The inhibitory concentration (IC₅₀) values of jatrorrhizine, palmatine and magnoflorine are 3.23, 3.45 and 1.25 µg/mL respectively. The concentration of maximum activity was selected for its effect on galactose-induced polyol accumulation in vitro. The percentage inhibition of galactose-induced polyol accumulation was 62.6, 58.8 and 27.7% in the presence of jatrorrhizine, palmatine and magnoflorine, respectively. Magnoflorine may be useful as lead compounds and new agents for AR inhibition.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036332852

Download citation: RISBibTeXText

PMID: 22294283

DOI: 10.1002/ptr.3721

Related references

Rat lens aldose reductase inhibitory activities of Coptis japonica root-derived isoquinoline alkaloids. Journal of Agricultural and Food Chemistry 50(24): 7013-7016, 2002

Comparative immunomodulation potential of Tinospora cordifolia (Willd.) Miers ex Hook. F., Tinospora sinensis (Lour.) Merrill and Tinospora cordifolia growing on Azadirachta indica A. Juss. Indian Journal of Experimental Biology 52(8): 808-813, 2014

Characterization of isoquinoline alkaloids, diterpenoids and steroids in the Chinese herb Jin-Guo-Lan (Tinospora sagittata and Tinospora capillipes) by high-performance liquid chromatography/electrospray ionization with multistage mass spectrometry. Rapid Communications in Mass Spectrometry 20(15): 2328-2342, 2006

Inhibition of lens aldose reductase by protopine alkaloids. Saudi Pharmaceutical Journal 4(1): 45-47, 1996

Alkaloids from tinospora cordifolia. Archiv der Pharmazie (Weinheim) 311(3): 251-256, 1981

Similarities and differences in the inhibition of rat lens aldose reductase and kidney aldehyde reductase by aldose reductase inhibitors. Investigative Ophthalmology & Visual Science 30(3 Suppl.): 193, 1989

Novel Spirosuccinimide Aldose Reductase Inhibitors Derived from Isoquinoline-1,3-diones: 2-[(4-Bromo-2-fluorophenyl)methyl]-6-fluorospiro[isoquinoline-4(11H),3'-pyrrolidine]-1,2',3,5'(2H)-tetrone and Congeners. 1. Journal of Medicinal Chemistry 37(13): 2043-2058, 1994

Novel spirosuccinimide aldose reductase inhibitors derived from isoquinoline-1,3-diones: 2-[(4-bromo-2-fluorophenyl)methyl]-6- fluorospiro[isoquinoline-4(1H),3'-pyrrolidine]-1,2',3,5'(2H)-tetrone and congeners. 1. Journal of Medicinal Chemistry 37(13): 2043-2058, 1994

Quantitative determination of protoberberine alkaloids in Tinospora cordifolia by RP-LC-DAD. Chromatographia: 3 4, 341-345, 2010

Aporphine alkaloids, clerodane diterpenes, and other constituents from Tinospora cordifolia. Fitoterapia 81(6): 485-489, 2010

Activation of aldose reductase in rat lens and metal-ion chelation by aldose reductase inhibitors and lipoic acid. Free Radical Research 25(4): 337-346, 1996

Purified rat lens aldose reductase. Polyol production in vitro and its inhibition by aldose reductase inhibitors. Biochemical Journal 240(1): 233-237, 1986

Immunological recognition of lens aldose reductase with antibodies prepared from human placental aldose reductase. Investigative Ophthalmology & Visual Science 20(3 Suppl.): 35, 1981

Rat lens aldose reductase: rapid purification and comparison with human placental aldose reductase. Experimental Eye Research 37(5): 467-474, 1983

Protoberberine alkaloids from callus and cell suspension cultures of Tinospora cordifolia. Pharmaceutical Biology 41(2): 81-86, 2003