+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Study of 5-hydroxymethylfurfural and its metabolite 5-sulfooxymethylfurfural on induction of colonic aberrant crypt foci in wild-type mice and transgenic mice expressing human sulfotransferases 1A1 and 1A2



Study of 5-hydroxymethylfurfural and its metabolite 5-sulfooxymethylfurfural on induction of colonic aberrant crypt foci in wild-type mice and transgenic mice expressing human sulfotransferases 1A1 and 1A2



Molecular Nutrition and Food Research 56(4): 593-600



It was reported that the Maillard product 5-hydroxymethylfurfural (HMF) initiates and promotes aberrant crypt foci (ACF) in rat colon. We studied whether 5-sulfooxymethylfurfural (SMF), an electrophilic and mutagenic metabolite of HMF, is able to induce ACF in two murine models. In the first model, FVB/N mice received four intraperitoneal administrations of SMF (62.5 or 125 mg/kg) or azoxymethane (10 mg/kg). Animals were killed 4-40 weeks after the last treatment. A total of 1064 ACF and five adenocarcinomas were detected in the azoxymethane-treated groups (20 animals), but none in the negative control and SMF-treated groups (35 and 50 animals, respectively). In the second model, HMF was administered via drinking water to wild-type FVB/N mice and transgenic mice carrying several copies of human sulfotransferase (SULT) 1A1 and 1A2 genes. HMF SULT activity was clearly elevated in cytosolic fractions of colon mucosa, liver and kidney of transgenic animals compared to wild-type mice and humans. The animals (six per group) received 134 and 536 mg HMF/kg/day for 12 weeks. HMF did not induce any ACF either in wild-type or transgenic animals. We found no evidence for an induction of ACF by HMF or its metabolite SMF in extensive studies in mice.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036343447

Download citation: RISBibTeXText

PMID: 22351042

DOI: 10.1002/mnfr.201100574


Related references

Toxicity studies with 5-hydroxymethylfurfural and its metabolite 5-sulphooxymethylfurfural in wild-type mice and transgenic mice expressing human sulphotransferases 1A1 and 1A2. Archives of Toxicology 86(5): 701-711, 2012

Development of 1,2-dimethylhydrazine-induced colonic aberrant crypt foci in PEPCK-IGF-II transgenic mice. Naunyn-Schmiedeberg's Archives of Pharmacology 365(Suppl. 1): R130, 2002

Plant tannins inhibit the induction of aberrant crypt foci and colonic tumors by 1,2-dimethylhydrazine in mice. Nutrition and Cancer 39(1): 108-116, 2001

Formation of DNA adducts in wild-type and transgenic mice expressing human sulfotransferases 1A1 and 1A2 after oral exposure to furfuryl alcohol. Mutagenesis 30(5): 643-649, 2015

Plasma membrane-associated sialidase (NEU3) promotes formation of colonic aberrant crypt foci in azoxymethane-treated transgenic mice. Cancer Science 100(4): 588-594, 2009

Intestinal carcinogenesis of two food processing contaminants, 2-amino-1-methyl-6-phenylimidazo4,5-bpyridine and 5-hydroxymethylfurfural, in transgenic FVB min mice expressing human sulfotransferases. 2011

Intestinal carcinogenesis of two food processing contaminants, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and 5-hydroxymethylfurfural, in transgenic FVB min mice expressing human sulfotransferases. Molecular Carcinogenesis 51(12): 984-992, 2012

Ethanol and 4-methylpyrazole increase DNA adduct formation of furfuryl alcohol in FVB/N wild-type mice and in mice expressing human sulfotransferases 1A1/1A2. Carcinogenesis 37(3): 314-319, 2016

Overexpression of PPARdelta and cyclin D1 in dysplastic aberrant crypt foci and in adenomas, but not in hyperplastic aberrant crypt foci in Apc Min/+ mice. Toxicology Letters (Shannon) 144(Suppl 1): s122, 2003

Comparative DNA adduct formation and induction of colonic aberrant crypt foci in mice exposed to 2-amino-9H-pyrido[2,3-b]indole, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline, and azoxymethane. Environmental and Molecular Mutagenesis 57(2): 125-136, 2016

Resveratrol from transgenic alfalfa for prevention of aberrant crypt foci in mice. Nutrition and Cancer 62(3): 351-361, 2010

Mice overexpressing progastrin are predisposed for developing aberrant colonic crypt foci in response to AOM. American Journal of Physiology. Gastrointestinal and Liver Physiology 278(3): G390-G399, 2000

Antitumor activity of the β-glucan paramylon from Euglena against preneoplastic colonic aberrant crypt foci in mice. Food and Function 4(11): 1685-1690, 2013

Effect of age on susceptibility to azoxymethane-induced colonic aberrant crypt foci formation in C57BL/6JNIA mice. Journals of Gerontology. Series A Biological Sciences and Medical Sciences 58(5): B400-B405, 2003

Vitamin E supplementation does not alter azoxymethane-induced colonic aberrant crypt foci formation in young or old mice. Journal of Nutrition 133(2): 528-532, 2003