+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

The photoprotective protein PsbS exerts control over CO(2) assimilation rate in fluctuating light in rice



The photoprotective protein PsbS exerts control over CO(2) assimilation rate in fluctuating light in rice



Plant Journal 71(3): 402-412



A direct impact of chloroplastic protective energy dissipation (qE) on photosynthetic CO(2) assimilation has not been shown directly in plants in the absence of photoinhibition. To test this empirically we transformed rice to possess higher (overexpressors, OE) and lower (RNA interference, RNAi) levels of expression of the regulatory psbS gene and analysed CO(2) assimilation in transformants in a fluctuating measurement light regime. Western blots showed a several-fold difference in levels of PsbS protein between RNAi and OE plants with the wild type (WT) being intermediate. At a growth light intensity of 600 μmol m(-2) sec(-1) , the carboxylation capacity, electron transport capacity and dark adapted F(v)/F(m) (ratio of variable to maximum fluorescence) were inhibited in RNAi plants compared with WT and OE. The PsbS content had a significant impact on qE (measured here as non-photochemical quenching, NPQ) but the strongest effect was observed transiently, immediately following the application of light. This capacity for qE was several-fold lower in RNAi plants and significantly higher in OE plants during the first 10 min of illumination. At steady state the differences were reduced: notably at 500 μmol m(-2) sec(-1) all plants had the same NPQ values regardless of PsbS content. During a series of light-dark transitions the induction of CO(2) assimilation was inhibited in OE plants, reducing integrated photosynthesis during the light period. We conclude that the accumulation of PsbS and the resultant qE exerts control over photosynthesis in fluctuating light, showing that optimization of photoprotective processes is necessary for maximum photosynthetic productivity even in the absence of photoinhibitory stress.

(PDF emailed within 0-6 h: $19.90)

Accession: 036363475

Download citation: RISBibTeXText

PMID: 22413771

DOI: 10.1111/j.1365-313X.2012.04995.x


Related references

Physiological functions of PsbS-dependent and PsbS-independent NPQ under naturally fluctuating light conditions. Plant & Cell Physiology 55(7): 1286-1295, 2015

Arabidopsis plants lacking PsbS protein possess photoprotective energy dissipation. Plant Journal 61(2): 283-289, 2010

Restoration of rapidly reversible photoprotective energy dissipation in the absence of PsbS protein by enhanced DeltapH. Journal of Biological Chemistry 286(22): 19973-19981, 2011

Elucidation of Photoprotective Mechanisms of PSI Against Fluctuating Light photoinhibition. Plant & Cell Physiology 57(7): 1405-1414, 2016

Photosynthesis of marine algae in fluctuating light i. adjustment of rate in constant and fluctuating light regimes. Functional Ecology 1(4): 377-386, 1987

QE-type Photoprotective Energy Dissipation without PsbS. 2011

Allocation of absorbed light energy in PSII to thermal dissipations in the presence or absence of PsbS subunits of rice. Plant & Cell Physiology 52(10): 1822-1831, 2012

PsbS is a high light inducible protein important for plant fitness. Photosynthesis Research 69(1-3): 31, 2001

Expression of the early light-induced protein but not the PsbS protein is influenced by low temperature and depends on the developmental stage of the plant in field-grown pea cultivars. Plant cell and environment 26(2): 245-253, 2003

The role of the PsbS protein in the protection of photosystems I and II against high light in Arabidopsis thaliana. Biochimica et Biophysica Acta 1817(12): 2158-2165, 2012

Chlamydomonas reinhardtii PsbS Protein Is Functional and Accumulates Rapidly and Transiently under High Light. Plant Physiology 171(4): 2717-2730, 2016

Regulation of photosynthetic light harvesting involves intrathylakoid lumen pH sensing by the PsbS protein. Journal of Biological Chemistry 279(22): 22866-22874, 2004

Studies on the light-curves of carbon assimilation of rice plants - the interrelationships among the light curves, plant type and maximizing the yield of rice. Bulletin of the National Institute of Agricultural Sciences, A (19): 1-100, 1972

Photosynthetic light-use efficiency in rice (Oryza sativa L.) leaf under light with fluctuating intensities at two different ambient humidities. Plant Production Science 3(2): 79-83, 2000

Acceleration of cyclic electron flow in rice plants (Oryza sativa L.) deficient in the PsbS protein of Photosystem II. Plant Physiology and Biochemistry 84: 233-239, 2015