EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii


Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii



Applied and Environmental Microbiology 78(10): 3759-3762



ISSN/ISBN: 0099-2240

PMID: 22407679

DOI: 10.1128/aem.05659-11

The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036390937

Download citation: RISBibTeXText

Related references

Cloning, heterologous expression, and enzymatic characterization of a thermostable glucoamylase from Talaromyces emersonii. Protein Expression and Purification 26(1): 1-8, 2002

cDNA cloning and expression of a Talaromyces emersonii β-glucosidase determinant in Escherichia coli. Biochimica et Biophysica Acta - Gene Structure and Expression 1049(1): 27-32, 1990

CDNA cloning and expression of a Talaromyces emersonii beta-glucosidase determinant in Escherichia coli. Biochimica et Biophysica Acta 1049(1): 27-32, 1990

CDNA cloning and expression of a Talaromyces emersonii amylase encoding genetic determinant in Escherichia coli. Biotechnology letters 14(12): 1109-1114, 1992

Evaluation of three thermostable fungal endo- beta -glucanases from Talaromyces emersonii for brewing and food applications. Process Biochemistry 40(5): 1741-1748, 2005

Cloning and expression of a thermostable alpha-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris. Journal of Microbiology and Biotechnology 20(12): 1653-1663, 2010

Cloning and overexpression of thermostable DNA polymerase gene in Escherichia coli. Chinese Journal of Biotechnology 11(3): 185-191, 1995

Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii. European Journal of Biochemistry 271(22): 4495-4506, 2004

Stereoselective Production of (+)-trans-Chrysanthemic Acid by a Microbial Esterase: Cloning, Nucleotide Sequence, and Overexpression of the Esterase Gene of Arthrobacter globiformis in Escherichia coli. Applied and Environmental Microbiology 62(4): 1491, 1996

Stereoselective production of (+)-trans-chrysanthemic acid by a microbial esterase: cloning, nucleotide sequence, and overexpression of the esterase gene of Arthrobacter globiformis in Escherichia coli. Applied and Environmental Microbiology 61(9): 3208-3215, 1995

Mitochondrial malate dehydrogenase from the thermophilic, filamentous fungus Talaromyces emersonii. Purification of the native enzyme, cloning and overexpression of the corresponding gene. European Journal of Biochemistry 271(15): 15-26, 2004

Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia coli. FEMS Microbiology Letters 162(1): 185-191, 1998

Molecular cloning of extremely thermostable esterase gene from hyperthermophilic archaeon Pyrococcus furiosus in Escherichia coli. Biotechnology and Bioengineering 57(5): 624-629, Ch 5, 1998

Molecular cloning of extremely thermostable esterase gene from hyperthermophilic archaeon Pyrococcus furiosus in Escherichia coli. Biotechnology and Bioengineering 57(5): 624-629, 1998

Expression and characterization of a thermostable acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis involved in the degradation of insoluble cellulose acetate. Bioscience Biotechnology and Biochemistry 77(12): 2495-2498, 2013