+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Xenobiotic- and jasmonic acid-inducible signal transduction pathways have become interdependent at the Arabidopsis CYP81D11 promoter



Xenobiotic- and jasmonic acid-inducible signal transduction pathways have become interdependent at the Arabidopsis CYP81D11 promoter



Plant Physiology 159(1): 391-402



Plants modify harmful substances through an inducible detoxification system. In Arabidopsis (Arabidopsis thaliana), chemical induction of the cytochrome P450 gene CYP81D11 and other genes linked to the detoxification program depends on class II TGA transcription factors. CYP81D11 expression is also induced by the phytohormone jasmonic acid (JA) through the established pathway requiring the JA receptor CORONATINE INSENSITIVE1 (COI1) and the JA-regulated transcription factor MYC2. Here, we report that the xenobiotic- and the JA-dependent signal cascades have become interdependent at the CYP81D11 promoter. On the one hand, MYC2 can only activate the expression of CYP81D11 when both the MYC2- and the TGA-binding sites are present in the promoter. On the other hand, the xenobiotic-regulated class II TGA transcription factors can only mediate maximal promoter activity if TGA and MYC2 binding motifs, MYC2, and the JA-isoleucine biosynthesis enzymes DDE2/AOS and JAR1 are functional. Since JA levels and degradation of JAZ1, a repressor of the JA response, are not affected by reactive chemicals, we hypothesize that basal JA signaling amplifies the response to chemical stress. Remarkably, stress-induced expression levels were 3-fold lower in coi1 than in the JA biosynthesis mutant dde2-2, [corrected] revealing that COI1 can contribute to the activation of the promoter in the absence of JA. Moreover, we show that deletion of the MYC2 binding motifs abolishes the JA responsiveness of the promoter but not the responsiveness to COI1. These findings suggest that yet unknown cis-element(s) can mediate COI1-dependent transcriptional activation in the absence of JA.

(PDF emailed within 0-6 h: $19.90)

Accession: 036394254

Download citation: RISBibTeXText

PMID: 22452854

DOI: 10.1104/pp.112.194274


Related references

Xenobiotic- and Jasmonic Acid-Inducible Signal Transduction Pathways Have Become Interdependent at the Arabidopsis Cyp81D11 Promoter. Plant Physiology 159(1): 391-402, 2012

Abscisic acid and jasmonic acid activate wound-inducible genes in potato through separate, organ-specific signal transduction pathways. Plant Journal 11(4): 773-782, 1997

Jasmonic acid-dependent and -independent wound signal transduction pathways are differentially regulated by Ca2+/calmodulin in Arabidopsis thaliana. Molecular and General Genetics 258(4): 412-419, 1998

Reversible protein phosphorylation regulates jasmonic acid-dependent and -independent wound signal transduction pathways in Arabidopsis thaliana. Plant Journal 13(2): 153-165, 1998

Jasmonic acid in wound signal transduction pathways. Physiologia Plantarum. 101(4): 740-745,., 1997

Jasmonic acid-dependent and -independent wound signal transduction pathways are differentially regulated by Ca2+. Molecular and general genetics: MGG 258(4): 412-419, 1998

Signal transduction downstream of salicylic and jasmonic acid in herbivory-induced parasitoid attraction by Arabidopsis is independent of JAR1 and NPR1. Plant cell and environment 26(9): 1541-1548, 2003

A knock-out mutation in allene oxide synthase results in male sterility and defective wound signal transduction in Arabidopsis due to a block in jasmonic acid biosynthesis. Plant journal: for cell and molecular biology 31(1): 1-12, 2002

Arabidopsis casein kinase 1-like 2 involved in abscisic acid signal transduction pathways. Journal of Plant Interactions 9(1): 19-25, 2014

Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter. European Journal of Biochemistry 270(24): 4876-4886, 2003

Wounding Changes the Spatial Expression Pattern of the Arabidopsis Plastid -3 Fatty Acid Desaturase Gene (Fad7) through Different Signal Transduction Pathways. The Plant Cell 9(10): 1701-1712, 1997

Wounding changes the spatial expression pattern of the Arabidopsis plastid q-3 fatty acid desaturase gene (FAD7) through different signal transduction pathways. Plant Cell 9: 01-12, 1997

Arabidopsis Functional Genomics || Two-Component Signal Transduction Pathways in Arabidopsis. Plant Physiology 129(2): 500-515, 2002

Wounding changes the spatial expression pattern of the arabidopsis plastid omega-3 fatty acid desaturase gene (FAD7) through different signal transduction pathways. Plant Cell 9(10): 1701-1712, 1997

Genetic analysis of osmotic and cold stress signal transduction in Arabidopsis: interactions and convergence of abscisic acid-dependent and abscisic acid-independent pathways. Plant Cell 9(11): 1935-1949, 1997