+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Hyperthermophilic archaea produce membrane vesicles that can transfer DNA

Environmental Microbiology Reports 5(1): 109-116

Hyperthermophilic archaea produce membrane vesicles that can transfer DNA

Thermococcales are hyperthermophilic archaea found in deep-sea hydrothermal vents. They have been recently reported to produce membrane vesicles (MVs) into their culture medium. Here, we have characterized the mode of production and determined the biochemical composition of MVs from two species of Thermococcales, Thermococcus gammatolerans and Thermococcus kodakaraensis. We observed that MVs are produced by a budding process from the cell membrane reminiscent of ectosome (microparticle) formation in eukaryotes. MVs and cell membranes from the same species have a similar protein and lipid composition, confirming that MVs are produced from cell membranes. The major protein present in cell membranes and MVs of both species is the oligopeptide binding protein OppA. This protein is also abundant in MVs from cells grown in minimal medium, suggesting that OppA could be involved in processes other than peptides scavenging. We have previously shown that MVs from Thermococcales harbour DNA and protect DNA against thermodegradation. Here, we show that T. kodakaraensis cells transformed with the shuttle plasmid pLC70 release MVs harbouring this plasmid. Notably, these MVs can be used to transfer pLC70 into plasmid-free cells, suggesting that MVs could be involved in DNA transfer between cells at high temperature.

(PDF same-day service: $19.90)

Accession: 036395657

PMID: 23757139

DOI: 10.1111/j.1758-2229.2012.00348.x

Related references

Membrane vesicles, nanopods and/or nanotubes produced by hyperthermophilic archaea of the genus Thermococcus. Biochemical Society Transactions 41(1): 436-442, 2013

Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima: unusual regulatory properties in hyperthermophilic archaea. Journal of Biological Chemistry 278(28): 25417-25427, 2003

Virus-like vesicles and extracellular DNA produced by hyperthermophilic archaea of the order Thermococcales. Research in Microbiology 159(5): 390-399, 2008

Evidence of recent lateral gene transfer among hyperthermophilic Archaea. Molecular Microbiology 38(4): 684-693, November, 2000

Evidence for lateral gene transfer from genomic analysis of Hyperthermophilic archaea. Abstracts of the General Meeting of the American Society for Microbiology 100: 378, 2000

Gram-positive bacteria produce membrane vesicles: proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles. Proteomics 9(24): 5425-5436, 2010

Catalytic and regulatory roles of divalent metal cations on the phosphoryl-transfer mechanism of ADP-dependent sugar kinases from hyperthermophilic archaea. Biochimie 94(2): 516-524, 2012

Thermoadaptation of a mesophilic hygromycin B phosphotransferase by directed evolution in hyperthermophilic Archaea: Selection of a stable genetic marker for DNA transfer into Sulfolobus solfataricus. Extremophiles 5(3): 153-159, June, 2001

Membrane vesicles prepared by different procedures from Escherichia coli: orientation of membrane vesicles and coupling of energy to transport into membrane vesicles. Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme 20(12): 1111-1122, 1975

DNA topoisomerases VI from hyperthermophilic archaea. Methods in Enzymology 334: 172-179, 2001