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Construction of a genetically engineered microorganism for phenanthrene biodegradation



Construction of a genetically engineered microorganism for phenanthrene biodegradation



Journal of Basic Microbiology 53(2): 188-194



The bacterium Pseudomonas sp. CGMCC2953, isolated from oil-polluted soil, was used as a recipient for a biodegradative gene encoding catechol 2,3-dioxygenase (C23O), which was successfully cloned into the plasmid pK4 derived from pRK415 with a broad host range. The apparent phenanthrene biodegradation parameters of the recombinant microorganism (Pseudomonas sp. CGMCC2953-pK) were determined and compared with those of the wild type. As the key enzyme of phenanthrene degradation, C23O, could be expressed constitutively in the recombinant strain, Pseudomonas sp. CGMCC2953-pK showed an increased ability to degrade phenanthrene. The excessive production of C23O in Pseudomonas sp. CGMCC2953-pK could serve as an effective approach to construct genetically engineered microorganisms for the bioremediation of environmental contaminations.

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Accession: 036397852

Download citation: RISBibTeXText

PMID: 22581686

DOI: 10.1002/jobm.201100322


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