+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Chlamydiaceae in riverine buffalo (Bubalus bubalis) and cows (Bos taurus) in Egypt with and without signs of reproductive disease



Chlamydiaceae in riverine buffalo (Bubalus bubalis) and cows (Bos taurus) in Egypt with and without signs of reproductive disease



New Zealand Veterinary Journal 60(4): 228-233



To obtain information and compare the prevalence of Chlamydiaceae in riverine buffalo (Bubalus bubalis) and cows (Bos taurus) in Egypt with and without clinical signs of reproductive disease. Vaginal swabs and blood samples were collected from animals attending Governmental Veterinary Clinics without (buffalo n=39, cows n=20) and with (buffalo n=63, cows n=53) signs of reproductive disease. Serum samples were tested for antibodies to Chlamydiaceae using complement fixation testing (CFT). Vaginal swabs were tested for Chlamydiaceae following inoculation into Vero cells and 6-day-old embryonated chicken eggs, using modified Giménez and immunoperoxidase staining, PCR analyses targeting the omp2 gene, and Restriction Fragment Length Polymorphism PCR (RFLP-PCR) for species identification. Antibodies to Chlamydiaceae were detected in 30/39 (77%) and 50/63 (79%) buffalo without and with signs of reproductive disease, respectively, and 10/20 (50%) and 39/53 (74%) of cows with and without signs of reproductive disease, respectively. Positive samples from PCR analysis were identified in 31/39 (79%) and 37/63 (59%) buffalo without and with signs of reproductive disease, respectively, and 12/20 (60%) and 46/53 (89%) of cows without and with signs of reproductive disease, respectively. Using RFLP-PCR, 57/68 (84%) of samples from buffalo, and 47/58 (81%) from cows, were identified as Chlamydophila psittaci and the reminder as Cp. abortus. From the CFT and PCR results there was no significant difference in the prevalence of positive samples between species, or between animals without or with signs of reproductive disease. The presence of anti-Chlamydiaceae antibodies in 77% of the animals with signs of reproductive disease and the detection of Chlamydiaceae in 72% of vaginal swabs of the animals suggest a pathogenic role by Chlamydiaceae in riverine buffalo and cows. The main Chlamydiaceae found in the genital tract of cattle in Egypt were Cp. psittaci and Cp. abortus. Chlamydophila spp. should be included in diagnostic algorithms for reproductive disorders, in order to assess the real burden of Chlamydophila associated disease in buffalo and cattle and to evaluate the potential value of vaccines.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036463172

Download citation: RISBibTeXText

PMID: 22712774

DOI: 10.1080/00480169.2012.668123


Related references

Reproductive endocrine profiles and follicular growth after estrus induction in the riverine water buffalo (Bubalus bubalis, 2n=50) and riverine-swamp hybrid buffalo (2n=49). Italian Journal of Animal Science 6: 622-625, 2007

Postpartum reproductive performance in buffalo cows (Bubalus bubalis). Revista Cientifica, Facultad de Ciencias Veterinarias, Universidad del Zulia 9(2): 116-123, 1999

Patho-anatomy of the female reproductive tract from non-productive buffalo-cows (Bubalus bubalis L.). Pantnagar journal of research 3(1): 95-97, 1978

Oestrous cycle duration, signs of oestrus and timing of ovulation in water buffalo cows (Bubalus bubalis) on Marajo Island, Northern Brazil. Zyklus, Dauer und Symptome der Brunst sowie Zeitpunkt der Ovulation bei Wasserbuffelkuhen Bubalus bubalis auf der Marajo Insel, Nordbrasilien: 99, 1983

Studies on the biometry of sperm of riverine buffalo bulls (Bubalus bubalis). Buffalo Bulletin 27(2): 192-195, 2008

QUALITY OF REFRIGERATED RAW MILK FROM BUFFALO COWS (Bubalus bubalis bubalis) IN DIFFERENT FARMS AND SEASONS IN BRAZIL. Ciência Animal Brasileira 18: e41815-e41815, 2017

Development of Sarcocystis cruzi in buffalo (Bubalus bubalis) and cattle (Bos taurus). Acta Veterinaria et Zootechnica Sinica 24(2): 185-192, 1993

A set of cattle microsatellite DNA markers for genome analysis of riverine buffalo (Bubalus bubalis). Animal Genetics 33(2): 149-154, 2002

Cyto genetic studies in water buffalo bubalus bubalis bos taurus bos indicus. Boletim de Industria Animal 23: 91-95, 1965, 1966

Evidence for bovine besnoitiosis in Egypt-first serosurvey of Besnoitia besnoiti in cattle and water buffalo (Bubalus bubalis) in Egypt. Tropical Animal Health and Production 46(3): 519-522, 2014

Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in Northern Vietnam. Veterinary Parasitology 166(3-4): 314-320, 2009

Comparisons of liveweight gain and changes in carcass composition between buffalo (Bubalus bubalis) and Bos taurus steers. Australian Journal of Agricultural Research 26(2): 415-422, 1975

Current status of river buffalo (Bubalus bubalis) gene map: comparison with the bovine (Bos taurus) map. Indian Journal of Dairy Science 53(3): 190-199, 2000

Cryogenic changes in proteases and antiprotease activities of buffalo (Bubalus bubalis) and cattle (Bos taurus) semen. Theriogenology 81(3): 396-402, 2014

In vitro production of cattle-water buffalo (Bos taurus - Bubalus bubalis) hybrid embryos. Zygote 10(2): 155-162, 2002