+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Natural transformation of Gallibacterium anatis



Natural transformation of Gallibacterium anatis



Applied and Environmental Microbiology 78(14): 4914-4922



Gallibacterium anatis is a pathogen of poultry. Very little is known about its genetics and pathogenesis. To enable the study of gene function in G. anatis, we have established methods for transformation and targeted mutagenesis. The genus Gallibacterium belongs to the Pasteurellaceae, a group with several naturally transformable members, including Haemophilus influenzae. Bioinformatics analysis identified G. anatis homologs of the H. influenzae competence genes, and natural competence was induced in G. anatis by the procedure established for H. influenzae: transfer from rich medium to the starvation medium M-IV. This procedure gave reproducibly high transformation frequencies with G. anatis chromosomal DNA and with linearized plasmid DNA carrying G. anatis sequences. Both DNA types integrated into the G. anatis chromosome by homologous recombination. Targeted mutagenesis gave transformation frequencies of >2 × 10(-4) transformants CFU(-1). Transformation was also efficient with circular plasmid containing no G. anatis DNA; this resulted in the establishment of a self-replicating plasmid. Nine diverse G. anatis strains were found to be naturally transformable by this procedure, suggesting that natural competence is common and the M-IV transformation procedure widely applicable for this species. The G. anatis genome is only slightly enriched for the uptake signal sequences identified in other pasteurellaceaen genomes, but G. anatis did preferentially take up its own DNA over that of Escherichia coli. Transformation by electroporation was not effective for chromosomal integration but could be used to introduce self-replicating plasmids. The findings described here provide important tools for the genetic manipulation of G. anatis.

(PDF emailed within 0-6 h: $19.90)

Accession: 036464346

Download citation: RISBibTeXText

PMID: 22582057

DOI: 10.1128/AEM.00412-12


Related references

Genetic relationships among avian isolates classified as Pasteurella haemolytica, 'Actinobacillus salpingitidis' or Pasteurella anatis with proposal of Gallibacterium anatis gen. nov., comb. nov. and description of additional genomospecies within Gallibacterium gen. nov. International Journal of Systematic and Evolutionary Microbiology 53(Pt 1): 275-287, 2003

Culture-independent identification and quantification of Gallibacterium anatis (G. anatis) by real-time quantitative PCR. Avian Pathology 45(5): 538-544, 2018

Detection of Gallibacterium anatis in layers by PCR and their histopathological observation in natural case. Journal Of Zhejiang University (agriculture And Life Sciences): 1, 109-114, 2010

Assessing pathogenicity of Gallibacterium anatis in a natural infection model: the respiratory and reproductive tracts of chickens are targets for bacterial colonization. Avian Pathology 42(6): 527-535, 2014

Pathogenesis of Gallibacterium anatis in a natural infection model fulfils Koch's postulates: 1. Folliculitis and drop in egg production are the predominant effects in specific pathogen free layers. Avian Pathology 43(5): 443-449, 2015

Pathogenesis of Gallibacterium anatis in a natural infection model fulfils Koch's postulates: 2. Epididymitis and decreased semen quality are the predominant effects in specific pathogen free cockerels. Avian Pathology 43(6): 529-534, 2016

Gallibacterium anatis bacteremia in a human. Journal of Clinical Microbiology 51(11): 3897-3899, 2014

Identification of a Hemagglutinin from Gallibacterium anatis. Current Microbiology 72(4): 450-456, 2016

Gallibacterium anatis pulmonary abscess. Medecine et Maladies Infectieuses 47(1): 74-76, 2016

In silico prediction of Gallibacterium anatis pan-immunogens. Veterinary Research 45: 80, 2016

Gallibacterium anatis-secreted metalloproteases degrade chicken IgG. Avian Pathology 34(5): 426-429, 2005

Bla OXA-10 and PSE-1 Genes Located on Class 1 Integrons in Gallibacterium anatis. Current Microbiology 2018, 2018

Identification of Gallibacterium anatis strains isolated in Peru. 2007

In vivo studies of Gallibacterium anatis infection in chickens. Avian Pathology 33(2): 145-152, 2004

Advance in a new avian disease Gallibacterium anatis infection. Chinese Veterinary Science: 2, 215-219, 2010