+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

A modified model of gentamicin induced renal failure in rats: toxicological effects of the iodinated X-ray contrast media ioversol and potential usefulness for toxicological evaluation of iodinated X-ray contrast media

A modified model of gentamicin induced renal failure in rats: toxicological effects of the iodinated X-ray contrast media ioversol and potential usefulness for toxicological evaluation of iodinated X-ray contrast media

Experimental and Toxicologic Pathology 65(5): 601-607

Contrast-induced nephropathy (CIN) remains a serious complication in patients exposed to iodinated X-ray contrast media (ICM). Animal models of CIN are useful to further understand the mechanisms involved, identify novel biomarkers and evaluate potential differences between ICM. The current investigation was undertaken to modify the rat-gentamicin model for potential usefulness for toxicological evaluation of ICM. A dose-range finding study (50, 60 and 70 mg/kg body weight (bw)) of gentamicin was conducted over 4 consecutive days. Based on the kidney histopathology findings, a gentamicin dose of 70 mg/kg bw was chosen to investigate whether ICM would cause further renal damage. Following gentamicin treatment, this group was given a single administration of ioversol (6 gI/kg bw). Blood and urine samples were taken from all animals 3 days before the start of the study and at the end of treatment. Serum and urinary creatinine, urinary N-acetyl-β-D-glucosaminidase (NAG), γ-glutamyl transferase (GGT), total protein, and urine cytology were evaluated as biomarkers of renal damage. Histopathological examination of kidneys was performed. Histopathological examination of the kidneys revealed vacuolation, dilatation, and necrosis of the proximal tubules in the gentamicin-ioversol treatment group. These changes were not seen in the gentamicin-only treatment groups. Data on GGT and urinary epithelial cells show clear differences between rats treated with gentamicin alone versus gentamicin plus ioversol. These findings show that the modified rat-gentamicin model was able to demonstrate the nephrotoxic effect of ioversol.

(PDF emailed within 0-6 h: $19.90)

Accession: 036465047

Download citation: RISBibTeXText

PMID: 22727913

DOI: 10.1016/j.etp.2012.06.003

Related references

Chromosome aberrations in rats injected with iodinated contrast media and antineoplastic drugs some toxicological considerations. Federation Proceedings 44(4): 925, 1985

The effects of the iodinated X-ray contrast media iodixanol, iohexol, iopromide, and ioversol on the rat kidney epithelial cell line NRK 52-E. Renal Failure 33(4): 426-433, 2011

Iodinated contrast media decrease renomedullary blood flow. A possible cause of contrast media-induced nephropathy. Advances in Experimental Medicine and Biology 645: 213-218, 2009

Recommendations of the European Society of Urogenital Radiology on the use of contrast media and the opportunity to use paramagnetic contrast in patients allergic to iodinated contrast media treated with endourology. Actas Urologicas Espanolas 26(10): 820, 2003

Renal failure and iodinated contrast media. A review. Jbr-Btr 82(2): 57-62, 2000

Reliability of experimental models of iodinated contrast media-induced acute renal failure. From methodological considerations to pathophysiology. Investigative Radiology 31(4): 230-241, 1996

Effects of non-ionic monomeric and dimeric iodinated contrast media on renal and systemic haemodynamics in rats. Fundamental & Clinical Pharmacology 14(1): 11-18, 2000

Prevention of nephrotoxicity by iodinated contrast media in patients with renal failure. Medicina Clinica 91(8): 281-282, 1988

Acute renal failure with iodinated contrast media in diabetic patients. Therapie 36(3): 351-357, 1981

Salvianolic Acid B Prevents Iodinated Contrast Media-Induced Acute Renal Injury in Rats via the PI3K/Akt/Nrf2 Pathway. Oxidative Medicine and Cellular Longevity 2016: 7079487, 2017

Anaerobic Transformation of the Iodinated X-ray Contrast Medium Iopromide, Its Aerobic Transformation Products, and Transfer to Further Iodinated X-ray Contrast Media. Environmental Science and Technology 52(15): 8309-8320, 2018

Nonionic iodinated contrast media: potential renal damage assessed with enzymuria. Radiology 183(1): 101-104, 1992

Cardiotrophin-1 administration prevents the renal toxicity of iodinated contrast media in rats. Toxicological Sciences 132(2): 493-501, 2013

Is Non-Contrast CT Adequate for the Evaluation of Hepatic Metastasis in Patients Who Cannot Receive Iodinated Contrast Media?. Plos One 10(7): E0134133, 2016

Distribution and relevance of iodinated X-ray contrast media and iodinated trihalomethanes in an aquatic environment. Chemosphere 184: 253-260, 2017