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Construction of a stable genetically engineered rhamnolipid-producing microorganism for remediation of pyrene-contaminated soil



Construction of a stable genetically engineered rhamnolipid-producing microorganism for remediation of pyrene-contaminated soil



World Journal of Microbiology and Biotechnology 28(9): 2783-2790



One rhamnolipid-producing bacterial strain named Pseudomonas aeruginosa BSFD5 was isolated and characterized. Its rhlABRI cassette including necessary genes for rhamnolipid synthesis was cloned and transformed into the chromosome of P. putida KT2440 by a new random transposon vector without introducing antibiotic-resistance marker, generating a genetically engineered microorganism named P. putida KT2440-rhlABRI, which could stably express the rhlABRI cassette and produce rhamnolipid at a yield of 1.68 g l(-1). In experiments using natural soil, it was shown that P. putida KT2440-rhlABRI could increase the dissolution of pyrene and thus promote its degradation by indigenous microorganisms. P. putida KT2440-rhlABRI thus demonstrated potential for enhancing the remediation of soils contaminated with polycyclic aromatic hydrocarbons.

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Accession: 036467440

Download citation: RISBibTeXText

PMID: 22806718

DOI: 10.1007/s11274-012-1088-0


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