+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transcriptome analysis of the entomopathogenic fungus Beauveria bassiana grown on cuticular extracts of the coffee berry borer (Hypothenemus hampei)



Transcriptome analysis of the entomopathogenic fungus Beauveria bassiana grown on cuticular extracts of the coffee berry borer (Hypothenemus hampei)



Microbiology 158(Pt 7): 1826-1842



The coffee berry borer (CBB; Hypothenemus hampei) is a major pest of coffee responsible for significant crop losses worldwide. The entomopathogenic fungus Beauveria bassiana represents a natural means of controlling this insect pest; however, little is known concerning the molecular determinants that contribute to the virulence of the fungus towards the CBB. In order to examine genes involved in insect virulence, two expressed sequence tag (EST) libraries, representing germinating conidia and growing hyphae/mycelia of B. bassiana cells grown on cuticular extracts of the CBB were constructed and analysed. In total, 4186 cDNA transcripts were obtained, which included 2141 from the cuticle-germinated conidia and 2045 from the cuticle-grown mycelium libraries, respectively. The average sequence length obtained was 470 bp and transcript assembly resulted in a set of 1271 and 1305 unique gene sequences for the conidial and mycelia libraries, respectively. Around 50 % of the sequences in each library could be annotated by gene ontology terms. An analysis of the two generated libraries as well as a previously reported EST library of B. bassiana grown on chitin was performed. Between the cuticle-germinated conidia and the cuticle-grown mycelia libraries, 322 unique gene sequences were shared, of which 90 % could be annotated, leaving 949 unique cuticle-germinated conidial genes and 983 unique growing hyphae/mycelia genes of which around 65 % were annotated. ESTs shared between the libraries indicated a basic response pattern for B. bassiana against H. hampei, which included genes implicated in pathogenicity. The expression profiles of four genes were evaluated with a cyclophilin, an alkaline-like serine protease and a mitogen-activated protein kinase (MAPK), showing elevated expression during initial phases of infection, i.e. conidia germinating on insect extracts. These data provide clues and gene candidates for further exploration concerning the biology and molecular mechanisms of entomopathogenicity by this fungus.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036479110

Download citation: RISBibTeXText

PMID: 22461485

DOI: 10.1099/mic.0.051664-0


Related references

Management of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. and its effect upon the coffee berry borer Hypothenemus hampei (Ferr.) and its parasitoid Cephalonomia stephanoderis Betrem. Revista Chapingo Serie Proteccion Vegetal 2(1): 39-43, 1995

Seasonal occurrence of the entomopathogenic fungus, Beauveria bassiana (Balsamo) on coffee berry borer, Hypothenemus hampei (Ferrari) (Scolytidae : Coleoptera) on Pulney hills, Tamil Nadu. Crop Research 34(1-3): 213-219, 2007

Occurrence of Beauveria bassiana in coffee berry borer Hypothenemus hampei in the State of Rondonia, Brazil Ocorrencia de Beauveria bassiana Bals Vuill em broca-do-cafe Hypothenemus hampei, Ferrari no Esatdo de Rondonia, Brasil. Acta Amazonica. Julho-Setembro; 323: 517-519, 2002

Infection of the coffee berry borer Hypothenemus hampei by Brazilian isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. Biocontrol Science & Technology 12(5): 631-635, 2002

Prevalence of naturally-occurring strains of Beauveria bassiana in populations of coffee berry borer Hypothenemus hampei on Hawai'i Island, with observations on coffee plant-H. hampei-B. bassiana interactions. Journal of Invertebrate Pathology 156: 54-72, 2018

Infection of the coffee berry borer Hypothenemus hampei (Coleoptera: Scolytidae) by Brazilian isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae (Deuteromycotina: Hyphomycetes). Biocontrol Science and Technology 12(5): 631-635, 2002

Beauveria bassiana strains selection for biological control of the coffee berry borer, Hypothenemus hampei Ferrari Coleoptera Scolytidae Selecao de isolados de Beauveria bassiana Para o controle biologico da Broca-do-Cafe, Hypothenemus hampei Ferrari Coleoptera Scolytidae. Neotropical Entomology. January; 341: 77-82, 2005

Enzymatic activity of Beauveria bassiana and Metarhizium anisopliae lipases and proteases and their relationship with pathogenicity to the coffee berry borer Hypothenemus hampei Coleoptera Scolytidae Actividad lipolitica y proteolitica de Beauveria bassiana y Metarhizium anisopliae y su relacion con la patogenicidad sobre Hypothenemus hampei Coleoptera Scolytidae. Revista Colombiana de Entomologia. Enero-Junio; 271-2: 61-65, 2001

Selection of Beauveria bassiana isolates for the control of the coffee berry borer Hypothenemus hampei. Revista Colombiana de Entomologia 23(1/2): 73-81, 1997

Characterization of some Beauveria bassiana isolates and their virulence toward the coffee berry borer Hypothenemus hampei. Journal of Invertebrate Pathology 67(2): 147-152, 1996

Genetic variability of Beauveria bassiana associated with the coffee berry borer Hypothenemus hampei and other insects. Mycological Research ember; 106(11): 1307-1314, 2002

Occurrence of Beauveria bassiana in coffee berry borer (Hypothenemus hampei) in the state of Rondonia, Brazil. Acta Amazonica 32(3): 517-519, 2002

Phylogenetic origins of African and Neotropical Beauveria bassiana s.l. pathogens of the coffee berry borer, Hypothenemus hampei. Journal of Invertebrate Pathology 93(1): 11-21, 2006

Biochemical and molecular characterization of isolates of Beauveria bassiana isolated from coffee berry borer, Hypothenemus hampei. Revista Colombiana de Entomologia 23(1/2): 51-57, 1997

Beauveria bassiana - a potential mycopesticide for the efficient control of coffee berry borer, Hypothenemus hampei (Ferrari) in India. Biocontrol Science and Technology 11(2): 251-260, 2001