+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Transport to late endosomes is required for efficient reovirus infection

Transport to late endosomes is required for efficient reovirus infection

Journal of Virology 86(16): 8346-8358

ISSN/ISBN: 0022-538X

PMID: 22674975

DOI: 10.1128/jvi.00100-12

Rab GTPases play an essential role in vesicular transport by coordinating the movement of various types of cargo from one cellular compartment to another. Individual Rab GTPases are distributed to specific organelles and thus serve as markers for discrete types of endocytic vesicles. Mammalian reovirus binds to cell surface glycans and junctional adhesion molecule-A (JAM-A) and enters cells by receptor-mediated endocytosis in a process dependent on β1 integrin. Within organelles of the endocytic compartment, reovirus undergoes stepwise disassembly catalyzed by cathepsin proteases, which allows the disassembly intermediate to penetrate endosomal membranes and release the transcriptionally active viral core into the cytoplasm. The pathway used by reovirus to traverse the endocytic compartment is largely unknown. In this study, we found that reovirus particles traffic through early, late, and recycling endosomes during cell entry. After attachment to the cell surface, reovirus particles and JAM-A codistribute into each of these compartments. Transfection of cells with constitutively active and dominant-negative Rab GTPases that affect early and late endosome biogenesis and maturation influenced reovirus infectivity. In contrast, reovirus infectivity was not altered in cells expressing mutant Rab GTPases that affect recycling endosomes. Thus, reovirus virions localize to early, late, and recycling endosomes during entry into host cells, but only those that traverse early and late endosomes yield a productive infection.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036510437

Download citation: RISBibTeXText

Related references

End13p/Vps4p is required for efficient transport from early to late endosomes in Saccharomyces cerevisiae. Journal of Cell Science 114(Pt 10): 1935-1947, 2001

Rab11 regulates the compartmentalization of early endosomes required for efficient transport from early endosomes to the trans-golgi network. Journal of Cell Biology 151(6): 1207-1220, 2000

NSF is required for transport from early to late endosomes. Journal of Cell Science 110: 2079-2087, 1997

Iga and Igb are required for efficient trafficking to late endosomes and to enhance antigen presentation. Journal of Immunology 162(11): 18-25, 1999

Ig alpha and Ig beta are required for efficient trafficking to late endosomes and to enhance antigen presentation. Journal of Immunology 162(11): 6518-6525, 1999

Efficient transport of avidin fusion proteins into late and early endosomes. Molecular Biology of the Cell 7(Suppl ): 452A, 1996

The golgin GCC88 is required for efficient retrograde transport of cargo from the early endosomes to the trans-Golgi network. Molecular Biology of the Cell 18(12): 4979-4991, 2007

Optimum length and flexibility of reovirus attachment protein σ1 are required for efficient viral infection. Journal of Virology 86(19): 10270-10280, 2012

Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes. Plos Biology 3(7): e233, 2005

Transport from Late Endosomes to Lysosomes, but Not Sorting of Integral Membrane Proteins in Endosomes, Depends on the Vacuolar Proton Pump. Journal of Cell Biology 130(4): 821-834, 1995

Murine infection with reovirus. IV. Late chronic disease and the induction of lymphoma after reovirus type 3 infection. British Journal of Experimental Pathology 47(4): 337-346, 1966

The porcine reproductive and respiratory syndrome virus requires trafficking through CD163-positive early endosomes, but not late endosomes, for productive infection. Archives of Virology 154(12): 1939-1943, 2009

Adenovirus VAI RNA is required for efficient translation of viral mRNAs at late times after infection. Cell 31(3 Pt 2): 543-551, 1982

Adenovirus vai rna is required for efficient translation of viral messenger rna at late times after infection. Cell 31(3 Part 2): 543-552, 1982

Cathepsin W Is Required for Escape of Influenza A Virus from Late Endosomes. Mbio 6(3): E00297, 2015