+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Prevalence and molecular epidemiology of human coronavirus HKU1 in patients with acute respiratory illness

Prevalence and molecular epidemiology of human coronavirus HKU1 in patients with acute respiratory illness

Journal of Medical Virology 85(2): 309-314

In 2005, human coronavirus HKU1 (HCoV-HKU1) was isolated and identified from a 71-year-old man with pneumonia in Hong Kong. To identify and classify genotypes of HCoV-HKU1 in Korea, a sensitive, specific, and quantitative real-time polymerase chain reaction (PCR) assay was developed and analyzed the sequences of HCoV-HKU1 isolated in Korea. A total of 1,985 respiratory specimens taken from patients with acute respiratory illness were tested for HCoV-HKU1 from January 2007 to May 2008. The major clinical symptoms associated with HCoV-HKU1 infection were examined statistically and sequence variations of the RNA-dependent RNA polymerase (RdRp), spike, and nucleocapsid genes were also analyzed. Fifty cases (2.5%) HCoV-HKU1 were identified by real-time PCR and viral loads ranged from 6.7 × 10(4) to 1.6 × 10(9)  copies/ml. The clinical symptoms of HCoV-HKU1 infection included rhinorrhea (72%), cough (64%), nasal congestion (56%), fever (32%), sputum (30%), sore throat (18%), chills (16%), postnasal discharge (14%), and tonsillar hypertrophy (10%). There was a seasonal distribution of HCoV-HKU1 infection, peaking in winter and spring. Both genotypes A and B were detected but no recombination between them was found. This is the first report on the identification and genotyping of HCoV-HKU1 as a causative agent of acute respiratory illness in Korea. The data suggest that at least two genotypes, A and B, of HCoV-HKU1 with scattered silent mutations were circulating in Korea from 2007 to 2008.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036655376

Download citation: RISBibTeXText

PMID: 23161446

DOI: 10.1002/jmv.23465

Related references

Molecular epidemiology and evolutionary histories of human coronavirus OC43 and HKU1 among patients with upper respiratory tract infections in Kuala Lumpur, Malaysia. Virology Journal 13: 33, 2016

Human respiratory coronavirus HKU1 versus other coronavirus infections in Italian hospitalised patients. Journal of Clinical Virology 38(3): 244-250, 2007

Comparative analysis of the activation of unfolded protein response by spike proteins of severe acute respiratory syndrome coronavirus and human coronavirus HKU1. Cell and Bioscience 4(1): 3, 2014

Molecular epidemiological and clinical features of coronavirus HKU1 in children with acute respiratory tract infection in Lanzhou. Zhonghua Er Ke Za Zhi 48(10): 744-747, 2010

Clinical features and molecular epidemiology of coronavirus-HKU1-associated community-acquired pneumonia. Hong Kong Medical Journal 15(Suppl. 9): 46-47, 2009

In silico analysis of ORF1ab in coronavirus HKU1 genome reveals a unique putative cleavage site of coronavirus HKU1 3C-like protease. Microbiology and Immunology 49(10): 899-908, 2005

Comparative analysis of 22 coronavirus HKU1 genomes reveals a novel genotype and evidence of natural recombination in coronavirus HKU1. Journal of Virology 80(14): 7136-7145, 2006

Prevalence and clinical characteristics of human CoV-HKU1 in children with acute respiratory tract infections in China. Journal of Clinical Virology 49(2): 126-130, 2010

Human Coronavirus Infections in Israel: Epidemiology, Clinical Symptoms and Summer Seasonality of HCoV-HKU1. Viruses 10(10):, 2018

Detection and clinical study on coronavirus HKU1 with acute lower respiratory tract infections of hospitalized children in Changsha. Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 25(1): 2-4, 2011

Human bocavirus, coronavirus, and polyomavirus detected among patients hospitalised with severe acute respiratory illness in South Africa, 2012 to 2013. Health Science Reports 1(8): E59, 2018

Epidemiology and clinical characteristics of human coronaviruses OC43, 229E, NL63, and HKU1: a study of hospitalized children with acute respiratory tract infection in Guangzhou, China. European Journal of Clinical Microbiology and Infectious Diseases 37(2): 363-369, 2018

Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome. Lancet 363(9403): 99-104, 2004

Molecular epidemiology of severe acute respiratory syndrome-associated coronavirus infections in Taiwan. Journal of Infectious Diseases 191(9): 1478-1489, 2005

Prevalence and genetic diversity analysis of human coronavirus OC43 among adult patients with acute respiratory infections in Beijing, 2012. Plos one 9(7): E100781, 2014