The effects of epidermal growth factor (EGF) on the in vitro development of isolated goat secondary follicles and the relative mRNA expression of EGF, EGF-R, FSH-R and P450 aromatase in cultured follicles

Silva, C.M.G.; Castro, S.V.; Faustino, L.R.; Rodrigues, G.Q.; Brito, I.R.; Rossetto, R.; Saraiva, M.V.A.; Campello, C.C.; Lobo, C.H.; Souza, C.E.A.; Moura, A.A.A.; Donato, M.A.M.; Peixoto, C.A.; Figueiredo, J.R.

Research in Veterinary Science 94(3): 453-461

2013


ISSN/ISBN: 1532-2661
PMID: 23270918
DOI: 10.1016/j.rvsc.2012.12.002
Accession: 036668369

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Abstract
The effects of varying concentrations of EGF were evaluated in terms of in vitro follicular development and the mRNA expression levels of EGF, EGF-R, FSH-R and P450 aromatase. After 6 days, the addition of 50 ng/mL of EGF to the culture medium increased the antrum formation rates in comparison to cultured control and after 18 days of culture produced oocytes with higher rates of meiosis resumption when compared to the other treatments (P<0.05). The daily follicular growth rates in presence of EGF (50 or 100) were increased in comparison to the cultured control (P<0.05). Treatment with EGF 50 stimulated the expression of EGF mRNA but reduced EGF-R mRNA expression and estradiol secretion as compared to the cultured control (P<0.05). After 18 days of culture, the mRNA levels for FSH-R and P450 aromatase were greater than those of the non-cultured controls (P<0.05). In conclusion, the effects of EGF treatment on the mRNA levels for EGF, EGF-R, FSH-R, and P450 aromatase varied according to the stage of follicle development.