+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Paraoxonase and arylesterase activities, lipid profile, and oxidative damage in experimental ischemic colitis model

Paraoxonase and arylesterase activities, lipid profile, and oxidative damage in experimental ischemic colitis model

Gastroenterology Research and Practice 2012: 979506-979506

Objective. In the present study, since PON1 is known as an HDL-associated antioxidant enzyme that inhibits the oxidative modification of LDL and oxidative stress plays a role in the pathogenesis of mesenteric ischemia, we investigated the changes in PON1 activity and lipid profile in an experimental ischemic colitis model. Methods. Forty male Wistar albino rats were divided into two groups: the control group (N = 15) and the experimental group (N = 25). All animals were anesthetized with ether and ketamine anesthesia to undergo a midline laparotomy. Ischemic colitis was induced by marginal vessel ligation in the splenic flexura (devascularization process). A sham laparotomy was performed in the control group. All animals were sacrificed on the seventh postoperative day. Oxidative stress marker (malonyldialdehyde, MDA), lipid profile, and paraoxonase (PON-1) and arylesterase activities were determined. Histopathological evaluation was done under light microscopy, after sectioning and staining with hematoxyline and eosin. Statistical analysis was conducted using Student's t-test and Mann-Whitney U test, and P < 0.05 was considered as statistically significant. Results. There was a significant decrease in both serum and tissue PON1 activity in ischemic colitis group (P < 0.01, for each). Similarly, arylesterase levels showed a parallel decrease in both tissue and serum of the experimental group (P < 0.01 and P < 0.001, retrospectively). MDA, an oxidative stress marker, was seen to increase in the experimental group (P < 0.01, tissue; P < 0.05, serum). In experimental group, there was a significant rise in serum total cholesterol and LDL levels (P < 0.001, for each). However, HDL level decreased significantly (P < 0.001). Triglycerides did not show any change between the groups (P > 0.05). Conclusions. PON1 and arylesterase play an important role in the pathophysiology of ischemic colitis.

(PDF emailed within 0-6 h: $19.90)

Accession: 036695418

Download citation: RISBibTeXText

PMID: 23197980

DOI: 10.1155/2012/979506

Related references

Serum lipid profile paraoxonase and arylesterase activities in psoriasis. Cell Biochemistry and Function 27(3): 176-180, 2009

The activities of serum paraoxonase and arylesterase and lipid profile in acute myeloid leukemia: preliminary results. European Review for Medical and Pharmacological Sciences 19(23): 4590-4594, 2016

Serum paraoxonase, arylesterase activities and oxidative status in patients with insomnia. European Review for Medical and Pharmacological Sciences 17(18): 2517-2522, 2014

Evaluation of oxidative stress, the activities of paraoxonase and arylesterase in patients with subclinic hypothyroidism. Acta Bio-Medica 82(3): 214-222, 2012

Evaluation of oxidative stress, the activities of paraoxonase and arylesterase in patients with subclinical hypothyroidism. Journal of Investigative Medicine 60(1): 23-28, 2013

Paraoxonase and arylesterase activities in patients with cardiac syndrome X, and their relationship with oxidative stress markers. Coronary Artery Disease 18(2): 89-95, 2007

Serum paraoxonase and arylesterase activities and oxidative stress levels in patients with SSRI intoxication. Journal of Membrane Biology 247(1): 17-21, 2014

Medroxyprogesterone acetate, enoxaparin and pentoxyfylline cause alterations in lipid peroxidation, paraoxonase (PON1) activities and homocysteine levels in the acute oxidative stress in an experimental model of spinal cord injury. Acta Neurochirurgica 144(10): 1021-31; Discussion 1031, 2002

Paraoxonase and arylesterase activities in the serum of two hyperlipoproteinaemic patients after repeated extracorporal lipid precipitation. Chemico Biological Interactions 119/120: 405-411, 1999

Acetaminophen intoxication is associated with decreased serum paraoxonase and arylesterase activities and increased lipid hydroperoxide levels. Human & Experimental Toxicology 33(11): 1134-1140, 2015

An evaluation of serum paraoxonase together with arylesterase activities and oxidative stress in children with intractable epilepsy: a cross-sectional study. Epilepsy Research 108(9): 1591-1596, 2015

Paraoxonase active site required for protection against LDL oxidation involves its free sulfhydryl group and is different from that required for its arylesterase/paraoxonase activities: Selective action of human paraoxonase allozymes Q and R. Arteriosclerosis Thrombosis and Vascular Biology 18(10): 1617-1624, 1998

Vitamin B6 supplementation improves oxidative stress and enhances serum paraoxonase/arylesterase activities in streptozotocin-induced diabetic rats. Thescientificworldjournal 2014: 351598-351598, 2015

Effects of soy isoflavones on carbon tetrachloride (CCL4)-induced liver damage and on the level of plasma paraoxonase with arylesterase activities. Saglk Bilimleri Dergisi, Firat Universitesi 19(4): 263-271, 2005