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Glycerol-3-phosphate Dehydrogenase 2 Is a Novel Factor H, FHL-1 and Plasminogen Binding Surface Protein of Candida albicans



Glycerol-3-phosphate Dehydrogenase 2 Is a Novel Factor H, FHL-1 and Plasminogen Binding Surface Protein of Candida albicans







Candida albicans utilizes human complement regulators, like Factor H, FHL-1 for immune evasion. In order to define the whole panel of fungal complement evasion molecules, C. albicans cell extract was absorbed to a Factor H coupled column. One 52 kDa protein was eluted and identified by mass spectrometry as the Glycerol-3-phosphate dehydrogenase 2. Consequently Gpd2 was recombinantly expressed and purified. Recombinant Gpd2 binds Factor H, FHL-1 mainly via SCR 7, and binds plasminogen via lysine residues. The three human regulators, when attached to candida Gpd2, are functionally active, which assisted in inactivation of complement or cleaved the extra-cellular matrix fibrinogen. Polyclonal Gpd2 anti-serum was generated and localized Gpd2 at the surface of C. albicans. In addition candida Gpd2 bound to human non-phagocytic cells, but not to phagocytic cells. Thus, candida Gpd2 is a novel fungal immune evasion protein, which binds several human regulators and in addition binds human cells.

Accession: 036720785

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