+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The simultaneous production of single-cell protein and a recombinant antibacterial peptide by expression of an antibacterial peptide gene in Yarrowia lipolytica

, : The simultaneous production of single-cell protein and a recombinant antibacterial peptide by expression of an antibacterial peptide gene in Yarrowia lipolytica. Process Biochemistry 48(2): 212-217

In this study, the DNA fragment encoding the N-terminus of scallop H2A was expressed in the marine-derived yeast Yarrowia lipolytica, which has a high protein content. After cultivation in PBB medium for 120h, the transformant producing the highest amount of antibacterial peptide, 29a, was obtained. The supernatant from cultures of 29a had killing activity against Vibrio harveyi, V. anguillarum, and V. parahaemolyticus. After purification, the molecular mass of the recombinant antibacterial peptide was 4.5kDa, and the purified recombinant antibacterial peptide was able to cause leakage of intracellular components from both whole and protoplast cells of V. parahaemolyticus. The results indicated that when the yeast transformant 29a was grown in YPD medium, PBB medium or hydrolysate of soybean meal containing ammonium sulfate, its cells still had a high protein content. Because this recombinant marine yeast both had a high protein content and produced the antibacterial peptide, it has high value-added applications. The DNA fragment encoding the N-terminus of scallop H2A was expressed in the marine derived Yarrowia lipolytica. The transformant 29a had the antibacterial activity against Vibrio spp and high protein content. The molecular mass of the recombinant antibacterial peptide was 4.5kDa. The purified recombinant antibacterial peptide could cause leakage of the bacteria.

(PDF same-day service: $19.90)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 036731379

DOI: 10.1016/j.procbio.2013.01.003

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Cutler, Anne, 1997: The syllable's role in the segmentation of stress languages. Language and Cognitive Processes 12(5-6): 839-845

Hulse, R.E.; Kunkler, P.E.; Fedynyshyn, J.P.; Kraig, R.P., 2004: Optimization of multiplexed bead-based cytokine immunoassays for rat serum and brain tissue. The ability to simultaneously quantify multiple signaling molecule protein levels from microscopic neural tissue samples would be of great benefit to deciphering how they affect brain function. This follows from evidence that indicates signaling m...

W H H, 1927: Influence of Insulin on Mortality from Diabetes. Canadian Medical Association Journal 17(1): 93-93

Gladkovich N.G.; Vorob'eva A.D.; Lushchekin V.S.; Shuleikina K.V., 1991: Morphometric parameters of neurons in reticular nuclei of the brain stem of a kitten. In the brain stem of 30-day old kittens three types of Golgi-stained neurons are distinguished: sparsely branched reticular neurons, densely branched arborescent neurons and multipolar giant neurons (according to the classification by Leontovitch)...

Przywojski, J., 2006: Opportunities for enterprise development in the odz region through the involvement of resources from the European Regional Development Fund within the framework of the Regional Operational Programme of odz Province for 2007-2013. This is a summary of a presentation made at the conference "Requirements for the restructuring of sugar factories in Poland", 19 September 2006, in odz, and deals in very general terms with the Regional Operational Programme's objec...

Templeman, N.M.; Beaudry, J.L.; Le Moine, C.M.R.; McClelland, G.B., 2011: Chronic hypoxia- and cold-induced changes in cardiac enzyme and gene expression in CD-1 mice. In mammals, environmental challenges often result in physical and metabolic cardiac remodeling (i.e., hypertrophy and a shift from lipid to carbohydrate oxidation). While chronic hypoxia and cold are both known to elicit cardiac changes, little is...

Spoerl, S.; Hendlmeier, C.; Hapfelmeier, A.; Wildgruber, M.; Schmid, R.M.; Peschel, C.; Saugel, B., 2016: Characteristics and outcome of patients presenting to the emergency department after autologous/allogeneic stem cell transplantation. Hematopoietic stem cell transplantations are still associated with a high risk of complications. Here, we characterize patients after autologous or allogeneic transplantation presenting to the emergency department and investigate factors associate...

Yuan HongXu; Shang HongSheng; Jing JinXue; Dang FenLing, 2003: Research on pathogenic characters of different virulence isolates of wheat take-all fungi. The pathogenic difference between isolates with different pathogenicity of Gaeumannomyces graminis var. tritici was studied with seminal roots of wheat seedlings. The results showed that the isolates with low virulence invaded the root slower than...

Veldhuis, J. D.; Sweinberg, S., 1981: Multi hormonal regulation of ornithine decarboxylase ec activity in highly differential porcine granulosa cells in vitro. Purified luteinizing hormone, but not follicle-stimulating hormone, elicited time- and dose-dependent stimulation of the cytosolic enzyme, ornithine decarboxylase, in highly differentiated, porcine granulosa cells maintained in vitro in a chemical...

Cermak, L.S.; Uhly, B.; Reale, L., 1980: Encoding specificity in the alcoholic Korsakoff patient. Brain and Language 11(1): 119-127