+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Image analysis of hyperchromatic crowded cell groups in SurePath cervical cytology

Image analysis of hyperchromatic crowded cell groups in SurePath cervical cytology

Cytopathology 24(2): 113-122

The discrimination of hyperchromatic crowded cell groups (HCCGs) in cervical cytology is a difficult and error-prone interpretive task. While the classic features of dyskaryosis are of undoubted value, the contribution of size, shape and colour intensity of HCCGs is less certain. This study employed morphometric analysis to determine whether HCCG area, shape and colour intensity are useful in categorising them. Seventy-five digital images from each of six categories of HCCG were subjected to image analysis. Ten variables relating to HCCG size, shape and colour intensity were assessed by discriminant function analysis. A further 28 cases were employed as a test set to determine the classification accuracy of the discriminant model. All samples were SurePath liquid-based cytology preparations. Nine of the 10 variables contributed significantly to the model (P<0.001) but no single variable had sufficient discriminative ability. Classification accuracy was highest for abnormal endocervical HCCGs and lowest for squamous metaplastic cells (64.0 vs. 17.3% correct classification rate). The accuracy of the model for distinguishing normal and abnormal HCCGs was 70.0%, which was significantly higher than chance (P<0.0001), but this reduced to 64.3% for the test cases, which was no better than chance (P>0.05). The area, shape and colour intensity of HCCGs, either alone or in combination, have little discriminative value. Practitioners and trainers should focus on the well-established features of dyskaryosis, such as chromatin pattern, nuclear membrane irregularities and group architecture. In terms of morphometric analysis, DNA ploidy and chromatin texture analysis may be more fruitful avenues of investigation.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036738801

Download citation: RISBibTeXText

PMID: 23342966

DOI: 10.1111/cyt.12040

Related references

Tennis racket cells in severe dyskaryosis in hyperchromatic crowded cell groups of SurePath(TM) cervical samples. Cytopathology 24(2): 136-138, 2013

Hyperchromatic crowded groups in cervical cytology--differing appearances and interpretations in conventional and ThinPrep preparations: a study from the College of American Pathologists Interlaboratory Comparison Program in Cervicovaginal Cytology. Archives of Pathology & Laboratory Medicine 130(3): 332-336, 2006

Hyperchromatic crowded cell groups in gynaecological liquid-based cytology samples. British Journal of Biomedical Science 67(3): 154-63; Quiz 165, 2010

Synchronous high-grade squamous intraepithelial lesion and adenocarcinoma in situ of cervix in a young woman presenting with hyperchromatic crowded groups in the cervical cytology specimen: report of a case. Diagnostic Cytopathology 36(11): 823-826, 2008

Pleomorphic spindle cells and hyperchromatic crowded groups in a cervical smear. Cytopathology 17(6): 396-398, 2006

Hyperchromatic crowded groups: pitfalls in pap smear diagnosis. American Journal of Clinical Pathology 114 Suppl: S36-S43, 2000

Evaluation and significance of hyperchromatic crowded groups (HCG) in liquid-based paps. Cytojournal 4: 2, 2007

P16(INK4a) and ProEx C immunostains facilitate differential diagnosis of hyperchromatic crowded groups in liquid-based Papanicolaou tests with menstrual contamination. Acta Cytologica 56(1): 55-61, 2012

Endometrial carcinoma detected with SurePath liquid-based cervical cytology: comparison with conventional cytology. Cytopathology 20(6): 380-387, 2010

Unsatisfactory rates vary between cervical cytology samples prepared using ThinPrep and SurePath platforms: a review and meta-analysis. Bmj Open 2(2): E000847, 2012

Isolation of RNA from cell lines and cervical cytology specimens stored in BD SurePath preservative fluid and downstream detection of housekeeping gene and HPV E6 expression using real time RT-PCR. Journal of Virological Methods 156(1-2): 138-144, 2009

Comparing SurePath, ThinPrep, and conventional cytology as primary test method: SurePath is associated with increased CIN II+ detection rates. Cancer Causes and Control 27(1): 15-25, 2016

Analytical performance of RNA isolated from BD SurePath™ cervical cytology specimens by the PreTect™ HPV-Proofer assay. Journal of Virological Methods 185(2): 199-203, 2012

Factors contributing to false-negative and potential false-negative cytology reports in SurePath™ liquid-based cervical cytology. Cytopathology 24(1): 39-43, 2013

Eight cases of malignant mixed Müllerian tumor (carcinosarcoma) of the uterus: findings in SurePath™ cervical cytology. Diagnostic Cytopathology 42(2): 165-169, 2014