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Advances of Modern Electrophoresis Methods with Laser-Induced Fluorescence

, : Advances of Modern Electrophoresis Methods with Laser-Induced Fluorescence.

Capillary electrophoresis (CE), especially free zone CE, offers a relatively simple separation with moderate selectivity based on the mobility of ions in solution. Laser-induced fluorescence (LIF) detection, an extremely sensitive technique, can be coupled with a variety of separation conditions to achieve sensitive and quantitative results. When these techniques are combined, CE/LIF provides the sensitivity and increased selectivity that makes trace level environmental analysis of fluorescent compounds possible at or below levels typical for GC/MS. We offer a panoramic review of the role of these tools in solving environmental and related analytical problems before providing a detailed experimental protocol.

Accession: 036752459

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Hoerr, V.; Ziebuhr, W.; Kozitskaya, S.; Katzowitsch, E.; Holzgrabe, U., 2007: Laser-induced fluorescence-capillary electrophoresis and fluorescence microplate reader measurement: two methods to quantify the effect of antibiotics. Putative antibiotic drugs have to be classified according to their bactericidal potential. Two new methods by means of fluorescence spectroscopy (FS) using a fluorescence microplate reader (FMR) and laser-induced fluorescence capillary electrophor...

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