+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vitro and in vivo evaluation of paclitaxel-loaded mesoporous silica nanoparticles with three pore sizes



In vitro and in vivo evaluation of paclitaxel-loaded mesoporous silica nanoparticles with three pore sizes



International Journal of Pharmaceutics 445(1-2): 12-19



In the present study, mesoporous silica nanoparticles (MSNs) with three pore size were manufactured by the etch method. A typical chemotherapeutic agent, paclitaxel (PTX) was loaded into these MSNs. The in vitro drug release behavior, the in vitro anti-tumor activity, the morphological apoptosis cell changes, cell apoptosis rate and pharmacokinetics were extensively evaluated to clarify the biomedical roles of these MSNs in the application of drug delivery. The results showed that paclitaxel-loaded MSNs not only demonstrated effective drug loading but also exhibited pore-size-dependent drug release performance in vitro. In addition, MSNs exhibited pore-size-dependent anti-tumor activity against breast cancer MCF-7 cells. The apoptosis mechanism study demonstrated that the percentage of early and late apoptosis of all PTX-loaded MSNs treated MCF-7 cells were significantly higher than that of free PTX, and additionally the percentage of apoptosis for PTX-loaded MSNs increased as the pore size of carriers enlarged. The pharmacokinetics results showed that PTX-loaded MSNs with the largest pore size exhibited the pharmacokinetic property similar to the PTX solution and the other drug loaded MSNs displayed sustained release behavior. These results demonstrate that MSNs could be a very promising drug delivery system for pore-size controllable drug release and enhancing the anti-tumor activity.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036752795

Download citation: RISBibTeXText

PMID: 23384728

DOI: 10.1016/j.ijpharm.2013.01.058


Related references

In vitro and in vivo evaluation of puerarin-loaded PEGylated mesoporous silica nanoparticles. Drug Development and Industrial Pharmacy 42(12): 2031-2037, 2016

Sustained-release study on Exenatide loaded into mesoporous silica nanoparticles: in vitro characterization and in vivo evaluation. Daru 25(1): 20, 2017

In vitro toxicity evaluation of lomefloxacin-loaded MCM-41 mesoporous silica nanoparticles. Drug and Chemical Toxicology 2019: 1-12, 2019

Development of duloxetine hydrochloride loaded mesoporous silica nanoparticles: characterizations and in vitro evaluation. Aaps Pharmscitech 16(4): 944-951, 2015

Investigation of the stability of Platinum nanoparticles incorporated in mesoporous silica with different pore sizes. Journal of Colloid and Interface Science 421: 22-26, 2014

Sub-150 nm mesoporous silica nanoparticles with tunable pore sizes and well-ordered mesostructure for protein encapsulation. Journal of Colloid and Interface Science 407: 236-242, 2013

In Vitro Evaluation of pH Responsive Doxazosin Loaded Mesoporous Silica Nanoparticles: A Smart Approach in Drug Delivery. Current Drug Delivery 13(4): 574-581, 2016

In vivo distribution and antitumor activity of doxorubicin-loaded N-isopropylacrylamide-co-methacrylic acid coated mesoporous silica nanoparticles and safety evaluation. European Journal of Pharmaceutics and Biopharmaceutics 85(3 Pt A): 406-412, 2013

Free paclitaxel loaded PEGylated-paclitaxel nanoparticles: preparation and comparison with other paclitaxel systems in vitro and in vivo. International Journal of Pharmaceutics 471(1-2): 525-535, 2014

Paclitaxel-loaded polymeric nanoparticles based on PCL-PEG-PCL: preparation, in vitro and in vivo evaluation. Journal of Controlled Release 152(Suppl. 1): E114-E116, 2011

Paclitaxel-loaded PEGylated PLGA-based nanoparticles: in vitro and in vivo evaluation. Journal of Controlled Release 133(1): 11-17, 2009

Controlled intracellular release of doxorubicin in multidrug-resistant cancer cells by tuning the shell-pore sizes of mesoporous silica nanoparticles. Acs Nano 5(12): 9788-9798, 2011

Paclitaxel-loaded polymeric nanoparticles combined with chronomodulated chemotherapy on lung cancer: In vitro and in vivo evaluation. International Journal of Pharmaceutics 516(1-2): 313-322, 2017

Ag-nanoparticle-loaded mesoporous silica: spontaneous formation of Ag nanoparticles and mesoporous silica SBA-15 by a one-pot strategy and their catalytic applications. Langmuir 28(10): 4768-4775, 2012

PEGylated poly(trimethylene carbonate) nanoparticles loaded with paclitaxel for the treatment of advanced glioma: in vitro and in vivo evaluation. International Journal of Pharmaceutics 420(2): 385-394, 2011