+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Novosphingobium barchaimii sp. nov., isolated from hexachlorocyclohexane-contaminated soil



Novosphingobium barchaimii sp. nov., isolated from hexachlorocyclohexane-contaminated soil



International Journal of Systematic and Evolutionary Microbiology 63(Pt 2): 667-672



A yellow-pigmented bacterial strain, designated LL02(T), was isolated from hexachlorocyclohexane-contaminated soil from Spolana Neratovice, a former Czech producer of lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL02(T) occupied a distinct phylogenetic position in the genus Novosphingobium and showed the highest sequence similarity with Novosphingobium resinovorum NCIMB 8767(T) (98.59 %). DNA-DNA relatedness between strain LL02(T) and its closest phylogenetic neighbours was <70 %, which indicated that strain LL02(T) represented a novel species of the genus Novosphingobium. The DNA G+C content of strain LL02(T) was 67.72±0 mol%. The major respiratory quinone was ubiquinone Q-10. The polar lipid profile of the isolate corresponded to those reported for other members of the genus Novosphingobium (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and sphingoglycolipids), thus supporting its classification in the genus. Spermidine was the major polyamine. The major fatty acids were summed feature 3 (consisting of C(16 : 1)ω7c and/or C(16 : 1)ω6c; 40.13 %), summed feature 8 (consisting of C(18 : 1)ω7c and/or C(18 : 1)ω6c; 31.09 %) and C(14 : 0) 2-OH (23.16 %). The results obtained from DNA-DNA hybridization and biochemical and physiological tests clearly distinguished the isolate from its closest phylogenetic neighbours. Thus, strain LL02(T) represents a novel species of the genus Novosphingobium, for which the name Novosphingobium barchaimii sp. nov. is proposed. The type strain is LL02(T) ( = CCM 7980(T)  = DSM 25411(T)).

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036772256

Download citation: RISBibTeXText

PMID: 22544801

DOI: 10.1099/ijs.0.039826-0


Related references

Novosphingobium panipatense sp. nov. and Novosphingobium mathurense sp. nov., from oil-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 59(Pt 1): 156-161, 2009

Corynebacteriumpollutisoli sp. nov., isolated from hexachlorocyclohexane-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 66(9): 3531-3537, 2016

Novosphingobium lindaniclasticum sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from an HCH dumpsite. International Journal of Systematic and Evolutionary Microbiology 63(Pt 6): 2160-2167, 2013

Pontibacter aurantiacus sp. nov. isolated from hexachlorocyclohexane (HCH) contaminated soil. International Journal of Systematic and Evolutionary Microbiology 67(5): 1400-1407, 2017

Edaphobacillus lindanitolerans gen. nov., sp. nov., isolated from hexachlorocyclohexane (HCH) contaminated soil. Journal of Basic Microbiology 53(9): 758-765, 2013

Sphingopyxis flava sp. nov., isolated from a hexachlorocyclohexane (HCH)-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 65(10): 3720-3726, 2015

Flavobacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 58(Pt 7): 1665-1669, 2008

Flavobacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 58(7): 1665-1669, 2008

Luteimonas tolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 66(4): 1851-1856, 2016

Sphingomonas indica sp. nov., isolated from hexachlorocyclohexane (HCH)-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 62(Pt 12): 2997-3002, 2012

Pontibacter ummariensis sp. nov., isolated from a hexachlorocyclohexane-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 66(2): 1080-1087, 2016

Microbacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 60(Pt 11): 2634-2638, 2010

Flavobacterium lindanitolerans sp nov, isolated from hexachlorocyclohexane-contaminated soil. International Journal Of Systematic And Evolutionary Microbiology: 7, 1665-1669, 2008

Parapedobacter indicus sp. nov., isolated from hexachlorocyclohexane-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 65(Pt 1): 129-134, 2015

Pontibacter indicus sp. nov., isolated from hexachlorocyclohexane-contaminated soil. International Journal of Systematic and Evolutionary Microbiology 64(Pt 1): 254-259, 2014