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Potential of citrus budlings originating in the Middle East as sources of citrus viroids



Potential of citrus budlings originating in the Middle East as sources of citrus viroids



Crop Protection 48: 13-15



A total of 101 citrus budlings belonging to 9 citrus cultivars which originated from 5 countries were collected from commercial nurseries in 2010. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) was used for detection of CEVd, CBLVd, HSVd, CDVd and CBCVd viroids from the budlings. Multiplex RT-PCR analysis, followed by sequencing of representative isolates, showed that 29, 28, 54, 68 and 79% of the citrus budlings are positive for CEVd, CBLVd, HSVd, CDVd and CBCVd viroids, respectively. Viroids were detected in citrus budlings originating in Oman, Lebanon, Syria, Egypt and Jordon. Viroids from Oman were found to share 94 100% nucleotide similarity with viroids from other parts of the world. Findings from this study provide evidence for circulation of citrus viroids via budlings originating in the Middle East. Strict quarantine measures and certification programs are required to reduce widespread distribution of citrus viroids via citrus budlings originating in these countries. Citrus budlings originating in the Middle East have a high level of contamination with citrus viroids. Citrus viroids from Oman share 94 100% nucleotide similarity with viroids from other parts of the world. Certification and quarantine programs are required to limit spread of citrus viroids in the Middle East.

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Accession: 036811003

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DOI: 10.1016/j.cropro.2013.02.006


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