+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection



Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection



International Journal of Infectious Diseases 17(6): E450-E453



Escherichia coli is the predominant pathogen causing urinary tract infection (UTI), the most common bacterial infectious disease encountered in clinical practice, accounting for significant morbidity and high medical costs. The severity of UTI produced by E. coli is due to the expression of a wide spectrum of virulence factors. In this study we evaluated the role of E. coli virulence determinants in the pathogenesis of UTI. A total of 90 uropathogenic E. coli strains were screened by PCR for the prevalence of seven virulence genes encoding type 1 fimbriae (fimH), pili associated with pyelonephritis (pap), S and F1C fimbriae (sfa and foc), afimbrial adhesins (afa), cytotoxic necrotizing factor (cnf), hemolysin (hly), and aerobactin (aer). The prevalence of genes coding for fimbrial adhesive systems was 68% for fimH, 41% for pap, and 34% for sfa/foc. The operons coding for afa afimbrial adhesins were identified in 20% of strains. The hly and cnf genes coding for toxins were amplified in 19% and 3% of strains, respectively. A prevalence of 52% was found for the aer gene. The various combinations of detected genes were designated as virulence patterns. The strains isolated from hospitalized patients displayed a great diversity of gene associations compared to those isolated from ambulatory patients. Our study showed that investigation of the bacterial pathogenicity associated with UTI may contribute to a better medical intervention.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036811479

Download citation: RISBibTeXText

PMID: 23510539

DOI: 10.1016/j.ijid.2013.01.025


Related references

Distribution of virulence genes and multiple drug-resistant patterns amongst different phylogenetic groups of uropathogenic Escherichia coli isolated from patients with urinary tract infection. Letters in Applied Microbiology 60(2): 148-154, 2015

Virulence-associated genes and drug susceptibility patterns of uropathogenic Escherichia coli isolated from patients with urinary tract infection. Infection and Drug Resistance Volume 12: 2039-2047, 2019

Association of some virulence genes with antibiotic resistance among uropathogenic Escherichia coli isolated from urinary tract infection patients in Alexandria, Egypt: A hospital-based study. Journal of Global Antimicrobial Resistance 2(2): 83-86, 2014

Phylotyping based virulence associated genes and drug susceptibility of Uropathogenic Escherichia coli in urinary tract infection patients. Journal of Microbiology and Biotechnology 2018, 2018

Prevalence of adhesive genes among uropathogenic Escherichia coli strains isolated from patients with urinary tract infection in Mangalore. Indian Journal of Medical Microbiology 32(2): 175-178, 2014

Virulence properties of uropathogenic Escherichia coli isolated from children with urinary tract infection in Korea. Genes and Genomics 40(6): 625-634, 2018

Virulence Factors of Uropathogenic and Commensal Escherichia Coli Pathotypes in Patients with Urinary Tract Infection. Archives of Disease in Childhood 97(Suppl 2): A263-A264, 2012

Distribution and genetic association of putative uropathogenic virulence factors iroN, iha, kpsMT, ompT and usp in Escherichia coli isolated from urinary tract infections in Japan. Journal of Urology 170(6 Pt 1): 2490-2493, 2003

Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model. Microbiology 155(Pt 5): 1634-1644, 2009

Prevalence of virulence factors and phylogenetic characterization of uropathogenic Escherichia coli causing urinary tract infection in patients with and without diabetes mellitus. Transactions of the Royal Society of Tropical Medicine and Hygiene 109(12): 769-774, 2015

The FimH Gene in Uropathogenic Escherichia coli Strains Isolated From Patients With Urinary Tract Infection. Jundishapur Journal of Microbiology 8(2): E17520, 2015

Uropathogenic Escherichia coli virulence and innate immune responses during urinary tract infection. Current Opinion in Microbiology 16(1): 100-107, 2013

Invasion and translocation of uropathogenic Escherichia coli isolated from urosepsis and patients with community-acquired urinary tract infection. European Journal of Clinical Microbiology and Infectious Diseases 37(5): 833-839, 2018

Phylogenetic group distributions, virulence factors and antimicrobial resistance properties of uropathogenic Escherichia coli strains isolated from patients with urinary tract infections in South Korea. Letters in Applied Microbiology 62(1): 84-90, 2016

Virulence profiles in uropathogenic Escherichia coli isolated from pregnant women and children with urinary tract abnormalities. Microbial Pathogenesis 52(5): 292-301, 2012