+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of enolases and aldolases as important fish allergens in cod, salmon and tuna: component resolved diagnosis using parvalbumin and the new allergens

Identification of enolases and aldolases as important fish allergens in cod, salmon and tuna: component resolved diagnosis using parvalbumin and the new allergens

Clinical and Experimental Allergy 43(7): 811-822

The majority of fish-allergic patients are sensitized to parvalbumin, known to be the cause of important IgE cross-reactivity among fish species. Little is known about the importance of fish allergens other than parvalbumin. The aim of this study was to characterize hitherto undefined fish allergens in three commonly consumed fish species, cod, salmon and tuna, and to evaluate their importance for in vitro IgE-diagnosis in addition to parvalbumin and fish gelatin. Sixty-two patients were diagnosed by clinical history, skin prick tests and specific IgE to fish extracts. Two new fish allergens from cod, salmon and tuna were identified by microsequencing. These proteins were characterized by immunoblot, ELISA and mediator release assay. Purified parvalbumin, enolase, aldolase and fish gelatin were used for quantification of specific IgE in ELISA. Parvalbumin and two other allergens of 50 and 40 kDa were detected in IgE-immunoblots of cod, salmon and tuna extracts by most patient sera. The 50 and 40 kDa proteins were identified as beta-enolase and fructose-bisphosphate aldolase A respectively. Both purified enzymes showed allergenic activity in the mediator release assay. Indeed, 72.6% of the patients were sensitized to parvalbumin, 20% of these had specific IgE to salmon parvalbumin only. IgE to enolases were found in 62.9% (0.5-95.0 kUA /L), to aldolases in 50.0% (0.4-26.0 kUA /L) and to fish gelatin in 19.3% (0.4-20.0 kUA /L) of the patients. Inter-species cross-reactivity, even though limited, was found for enolases and aldolases by IgE-inhibition ELISA. Fish enolase and aldolase have been identified as important new fish allergens. In fish allergy diagnosis, IgE to enolase and aldolase are especially relevant when IgE to parvalbumin are absent.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036824553

Download citation: RISBibTeXText

PMID: 23786287

DOI: 10.1111/cea.12117

Related references

Identification and characterization of allergens in two species of tuna fish. Annals of Allergy Asthma and Immunology 82(4): 395-400, 1999

Component-resolved diagnosis with recombinant allergens in patients with cherry allergy. Journal of Allergy and Clinical Immunology 110(1): 167-173, 2002

Diagnosis of Chenopodium album allergy with a cocktail of recombinant allergens as a tool for component-resolved diagnosis. Molecular Biology Reports 39(3): 3169-3178, 2012

Micro-arrayed wheat seed and grass pollen allergens for component-resolved diagnosis. Allergy 64(7): 1030-1037, 2009

Defining specific allergens for improved component-resolved diagnosis of shrimp allergy in adults. Molecular Immunology 112: 330-337, 2019

Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin). Journal of Allergy and Clinical Immunology 95(5): 962-969, 1995

Component-resolved diagnosis (CRD) of type I allergy with recombinant grass and tree pollen allergens by skin testing. Journal of Investigative Dermatology 113(5): 830-837, 1999

Spectrum of allergens for Japanese cedar pollinosis and impact of component-resolved diagnosis on allergen-specific immunotherapy. Allergology International 64(4): 312-320, 2015

Development of a light-initiated chemiluminescent assay for the quantitation of sIgE against egg white allergens based on component-resolved diagnosis. Analytical and Bioanalytical Chemistry 410(5): 1501-1510, 2018

Component-resolved diagnosis of pollen allergy based on skin testing with profilin, polcalcin and lipid transfer protein pan-allergens. Clinical and Experimental Allergy 39(11): 1764-1773, 2009

Component-resolved diagnosis of kiwifruit allergy with purified natural and recombinant kiwifruit allergens. Journal of Allergy and Clinical Immunology 125(3): 687-94 694.E1, 2010

Component-resolved diagnosis of house-dust mite allergy with purified natural and recombinant mite allergens. Clinical and Experimental Allergy 34(4): 597-603, 2004

Component-resolved in vitro diagnosis in carrot allergy: does the use of recombinant carrot allergens improve the reliability of the diagnostic procedure?. Clinical and Experimental Allergy 35(7): 970-978, 2005

Fish allergens at a glance: variable allergenicity of parvalbumins, the major fish allergens. Frontiers in Immunology 5: 179, 2014

Controlling the aggregation propensity and thereby digestibility of allergens by Maillardation as illustrated for cod fish parvalbumin. Journal of Bioscience and Bioengineering 111(2): 204-211, 2011