+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Proteomics of human seminal plasma: identification of biomarker candidates for fertility and infertility and the evolution of technology

Proteomics of human seminal plasma: identification of biomarker candidates for fertility and infertility and the evolution of technology

Molecular Reproduction and Development 80(5): 350-357

Proteomics is a research area that has developed rapidly in the last decade. It studies the large-scale characterization of the full protein components of a cell, a tissue, or a biological fluid. In the last decade, clinical proteomics has developed new technology and bioinformatics useful in identifying molecular markers of pathology; the next decade might be the era of proteomics. Seminal plasma (SP) represents a good sample for proteomic analysis in the evaluation of male fertility/infertility. SP is an acellular fluid conglomerate, comprised of contributions from the epididymis and accessory sexual glands. Human SP contains many proteins that are important to the successful fertilization of the oocyte by the spermatozoa. Proteomic studies have identified numerous seminal-specific proteins, and recent reports have provided a further understanding of their function with respect to male fertility. Upon further validation, these proteins may be useful in the clinical distinction between fertility and infertility. This article reviews the proteomic methods, such as one dimensional polyacrylamide gel electrophoresis (1D-PAGE), two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and mass spectrometry (MS), employed to detect human SP markers involved in fertility and infertility. As such, proteomic studies will help the development of new techniques to identify novel biomarkers for a better clinical diagnosis and treatment of male infertility.

(PDF emailed within 0-6 h: $19.90)

Accession: 036836965

Download citation: RISBibTeXText

PMID: 23559416

DOI: 10.1002/mrd.22178

Related references

Identification of biomarker candidates for fertility in spermatozoa of crossbred bulls through comparative proteomics. Theriogenology 119: 43-51, 2018

Identification of novel biomarker and therapeutic target candidates for acute intracerebral hemorrhage by quantitative plasma proteomics. Clinical Proteomics 14: 14, 2017

Identification of putative fertility markers in seminal plasma of crossbred bulls through differential proteomics. Theriogenology 82(9): 1254-62.E1, 2015

Differential proteomics of human seminal plasma: A potential target for searching male infertility marker proteins. Proteomics. Clinical Applications 6(3-4): 147-151, 2012

Proteomics of human cerebrospinal fluid: discovery and verification of biomarker candidates in neurodegenerative diseases using quantitative proteomics. Journal of Proteomics 74(4): 371-388, 2011

Isolation and identification of Concanavalin A binding glycoproteins from human seminal plasma: a step towards identification of male infertility marker proteins. Disease Markers 31(6): 379-386, 2012

Exploring the human seminal plasma proteome: an unexplored gold mine of biomarker for male infertility and male reproduction disorder. Journal of Reproduction and Infertility 16(2): 61-71, 2015

Anti-seminal plasma antibodies associated with infertility: II. Comparative investigation of human antibody binding to normo-, astheno-, and azoospermic seminal plasma antigens. American Journal of Reproductive Immunology 36(4): 204-210, 1996

Novel pancreatic beta cell-specific proteins: antibody-based proteomics for identification of new biomarker candidates. Journal of Proteomics 75(9): 2611-2620, 2012

Discovery of prognostic biomarker candidates of lacunar infarction by quantitative proteomics of microvesicles enriched plasma. Plos One 9(4): E94663, 2015

Seminal plasma as a source of prostate cancer peptide biomarker candidates for detection of indolent and advanced disease. Plos One 8(6): E67514, 2014

Proteomics of gliomas: initial biomarker discovery and evolution of technology. Neuro-Oncology 13(9): 926-942, 2011

Fractionation of the human plasma proteome for monoclonal antibody proteomics-based biomarker discovery 2: antigen identification by dot-blot array screening. Electrophoresis 34(20-21): 3064-3071, 2014

Targeted human cerebrospinal fluid proteomics for the validation of multiple Alzheimer's disease biomarker candidates. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 930: 129-135, 2014

Anti-seminal plasma antibodies associated with infertility: I. Serum antibodies against normozoospermic seminal plasma in patients with unexplained infertility. American Journal of Reproductive Immunology 36(4): 198-203, 1996