+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Electroelution of nucleic acids from polyacrylamide gels: a custom-made, agarose-based electroeluter

Electroelution of nucleic acids from polyacrylamide gels: a custom-made, agarose-based electroeluter

Analytical Biochemistry 437(1): 49-51

Polyacrylamide electrophoresis is routinely used for small-scale preparative and analytical separations. The incomparably high-resolution separations achieved by this technique, however, have not been widely exploited to the large-scale preparative isolation of biological molecules from contaminants, mainly because of difficulties in the recovery of the desired molecule from the gel matrix. Electroelution is an effective procedure applied for this purpose. However, commercially available, high-cost electroeluters are required for achieving high recovery yields. Here, we describe a custom-made electroeluter that combines low-cost, high-recovery yields, short times of electroelution, and convenience in the manipulation of sensitive samples.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036843595

Download citation: RISBibTeXText

PMID: 23499972

DOI: 10.1016/j.ab.2013.02.021

Related references

Rapid and inexpensive micro-electroelution of nucleic acid and protein from agarose and polyacrylamide gels. Biotechniques 13(3): 368-368, 1992

Electroelution on dna and protein from polyacrylamide and agarose gels. Biochemistry International 15(4): 687-692, 1987

Recovery of DNA from agarose and polyacrylamide gels: electroelution into dialysis bags. Csh Protocols 2006(1): -, 2006

Electrophoretic analysis of nucleic acids in agarose and polyacrylamide gels. Studii Si Cercetari de Inframicrobiologie 22(5): 459-474, 1971

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences of the United States of America 74(11): 4835-4838, 1977

An ethidium-acrylamide affinity medium for recovery of nucleic acids from free solution and from polyacrylamide and Agarose gels. Analytical Biochemistry 124(2): 414-420, 1982

A simple, inexpensive electroelution device for the recovery of nucleic acid fragments from agarose gels. Biotechniques 10(2): 159-160, 1991

SYBR Green I and SYBR Green II New ultrasensitive fluorescent stains for detecting picogram levels of nucleic acids in polyacrylamide or agarose gels. FASEB Journal 8(7): A1266, 1994

A paper-based electroelution system for protein recovery from stained sodium dodecyl sulfate-polyacrylamide gels. Analytical Biochemistry 381(2): 267-269, 2008

Electroelution of proteins from polyacrylamide gels. Methods in Molecular Biology 244: 339-343, 2004

Extraction of nucleic acids from agarose gels. Analytical Biochemistry 103(2): 264-271, 1980

Simultaneous electroelution and concentration of DNA fragments from agarose gels. Analytical Biochemistry 169(1): 194-196, 1988

Discontinuous polyacrylamide gradient agarose gels resolve a wide range of restriction fragments and optimize the efficiency of nucleic acid transfer. Analytical Biochemistry 173(2): 285-288, 1988

Hybridization of nucleic acids directly in agarose gels. Analytical Biochemistry 131(2): 365-372, 1983