+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Time-resolved flavin adenine dinucleotide fluorescence study of the interaction between immobilized glucose oxidase and glucose



Time-resolved flavin adenine dinucleotide fluorescence study of the interaction between immobilized glucose oxidase and glucose



Journal of Fluorescence 23(5): 947-955



Time-resolved fluorescence experiments have shown that flavin adenine dinucleotide (FAD) fluorescence emission of sol-gel immobilized glucose oxidase (GOD) exhibits a three-exponential decaying behaviour characterized by long- (about 2.0-3.0 ns), intermediate- (about 300 ps) and short- (less than 10 ps) lifetime, each one being characteristic of a peculiar conformational state of the FAD structure. In the present work time-resolved fluorescence is used to monitor FAD signals in the time interval immediately following the addition of glucose at various concentrations in order to detect the conformational changes occurring during the interaction between sol-gel immobilized GOD and glucose. The analysis of time-dependent fluorescence emission signal has shown that the FAD conformational state changes during the process from a configuration with a prevalence of the state characterized by the long lifetime to a configuration with increased contribution from the process with the intermediate lifetime. The time needed to complete this configuration change decreases with the concentration of added glucose. The results here reported indicate that time-resoled fluorescence can be extremely useful for a better understanding of solid phase biocatalysis that is particularly important in light of their clinical and biotechnological applications.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036845190

Download citation: RISBibTeXText

PMID: 23576005

DOI: 10.1007/s10895-013-1220-z


Related references

Immobilized glucose oxidase and D-amino acid oxidase: a convenient method for the purification of flavin adenine dinucleotide and its analogs. Analytical Biochemistry 95(1): 156-159, 1979

Electrochemical and enzymatic activity of flavin adenine dinucleotide and glucose oxidase immobilized by adsorption on carbon. Biotechnology and Bioengineering 26(11): 1364-1371, 1984

New insights into the analysis of the electrode kinetics of flavin adenine dinucleotide redox center of glucose oxidase immobilized on carbon electrodes. Langmuir 30(11): 3264-3273, 2014

Dynamical and structural properties of flavin adenine dinucleotide in aqueous solutions and bound to free and solgel immobilized glucose oxidase. Journal of Sol-Gel Science and Technology 82(1): 239-252, 2017

Glucose sensing by time-resolved fluorescence of sol-gel immobilized glucose oxidase. Sensors 11(4): 3483-3497, 2012

Kinetics of stacking interactions in flavin adenine dinucleotide from time-resolved flavin fluorescence. Photochemistry and Photobiology 40(6): 703-706, 1984

Time resolved fluorescence of flavin adenine dinucleotide. Febs Letters 44(1): 67-70, 1974

Reconstitution of flavin-adenine dinucleotide in the apoenzyme of glucose oxidase. Journal of Vitaminology 17(1): 24-31, 1971

Electrochemical and glucose oxidase coenzyme activity of flavin adenine dinucleotide covalently attached to glassy carbon at the adenine amino group. Biochimica et Biophysica Acta 838(1): 60-68, 1985

The relation between quaternary structure and the binding of flavin-adenine dinucleotide in glucose oxidase. Journal of Biochemistry 69(5): 969-972, 1971

The relationship between molecular conformation and the binding of flavin-adenine dinucleotide in glucose oxidase. Biochimica et Biophysica Acta 175(2): 365-379, 1969

The mechanism of binding of flavin-adenine dinucleotide to the apoenzyme of glucose oxidase and evidence for the involvement of multiple bonds. Biochimica et Biophysica Acta 175(2): 380-387, 1969

Amperometric Determination of Picomolar Levels of Flavin Adenine Dinucleotide by Cyclic Oxidation-Reduction in Apo-Glucose Oxidase System. Analytical Letters 13(13): 1157-1165, 1980

Resonance coherent anti-Stokes Raman scattering (CARS) spectra of flavin adenine dinucleotide, riboflavin binding protein and glucose oxidase. Biochemical and Biophysical Research Communications 83(1): 209-216, 1978

Glucose Determination by Means of Steady-state and Time-course UV Fluorescence in Free or Immobilized Glucose Oxidase. Sensors 7(11): 2612-2625, 2007