EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Single molecule FRET data analysis procedures for FRET efficiency determination: probing the conformations of nucleic acid structures


Methods 64(1): 36-42
Single molecule FRET data analysis procedures for FRET efficiency determination: probing the conformations of nucleic acid structures
Single molecule FRET microscopy is an attractive technique for studying structural dynamics and conformational diversity of nucleic acid structures. Some of its strengths are that it can follow structural changes on a fast time scale and identify conformation distributions arising from dynamic or static population heterogeneity. Here, we give a description of the experiment and data analysis procedures of this method and detail what parameters are needed for FRET efficiency calculation. Using single molecule FRET data obtained on G-quadruplex DNA structures that exhibit large conformation diversity, we illustrate that the shape of the FRET distribution changes depending on what parameters are included in the data analysis procedure.


Accession: 036846686

PMID: 23583888

DOI: 10.1016/j.ymeth.2013.04.001



Related references

BOBA FRET: bootstrap-based analysis of single-molecule FRET data. Plos One 8(12): E84157-E84157, 2014

Maximizing information content of single-molecule FRET experiments: multi-color FRET and FRET combined with force or torque. Chemical Society Reviews 43(4): 1007-1013, 2014

Future Perspective of Single-Molecule FRET Biosensors and Intravital FRET Microscopy. Biophysical Journal 111(6): 1103-1111, 2016

Integrated magnetic tweezers and single-molecule FRET for investigating the mechanical properties of nucleic acid. Methods 105: 16-25, 2016

Probing Protein Interactions Using GFP and FRET * Stage 2: Cell Preparation for FLIM-FRET Analysis. Csh Protocols 2006(1): -, 2006

Manipulating protein conformations by single-molecule AFM-FRET nanoscopy. Acs Nano 6(2): 1221-1229, 2012

Accurate single molecule FRET efficiency determination for surface immobilized DNA using maximum likelihood calculated lifetimes. Journal of Physical Chemistry. B 111(11): 2986-2990, 2007

LacI-DNA-IPTG loops: equilibria among conformations by single-molecule FRET. Journal of Physical Chemistry. B 117(16): 4713-4722, 2013

Molecular dynamics simulation of configurational ensembles compatible with experimental FRET efficiency data through a restraint on instantaneous FRET efficiencies. Journal of Computational Chemistry 35(32): 2319-2332, 2015

Single-Molecule FRET Reveals Three Conformations for the TLS Domain of Brome Mosaic Virus Genome. Biophysical Journal 109(12): 2625-2636, 2016