+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparison of human chordoma cell-kill for 290 MeV/n carbon ions versus 70 MeV protons in vitro

Comparison of human chordoma cell-kill for 290 MeV/n carbon ions versus 70 MeV protons in vitro

While the pace of commissioning of new charged particle radiation therapy facilities is accelerating worldwide, biological data pertaining to chordomas, theoretically and clinically optimally suited targets for particle radiotherapy, are still lacking. In spite of the numerous clinical reports of successful treatment of these malignancies with this modality, the characterization of this malignancy remains hampered by its characteristic slow cell growth, particularly in vitro. Cellular lethality of U-CH1-N cells in response to different qualities of radiation was compared with immediate plating after radiation or as previously reported using the multilayered OptiCellTM system. The OptiCellTM system was used to evaluate cellular lethality over a broad dose-depth deposition range of particle radiation to anatomically mimic the clinical setting. Cells were irradiated with either 290 MeV/n accelerated carbon ions or 70 MeV accelerated protons and photons and evaluated through colony formation assays at a single position or at each depth, depending on the system. There was a cell killing of approximately 20-40% for all radiation qualities in the OptiCellTM system in which chordoma cells are herein described as more radiation sensitive than regular colony formation assay. The relative biological effectiveness values were, however, similar in both in vitro systems for any given radiation quality. Relative biological effectiveness values of proton was 0.89, of 13-20 keV/ m carbon ions was 0.85, of 20-30 keV/ m carbon ions was 1.27, and >30 keV/ m carbon ions was 1.69. Carbon-ions killed cells depending on both the dose and the LET, while protons depended on the dose alone in the condition of our study. This is the first report and characterization of a direct comparison between the effects of charged particle carbon ions versus protons for a chordoma cell line in vitro. Our results support a potentially superior therapeutic value of carbon particle irradiation in chordoma patients.Carbon ion therapy may have an advantage for chordoma radiotherapy because of higher cell-killing effect with high LET doses from biological observation in this study.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 036851416

Download citation: RISBibTeXText

Related references

Comparison of human chordoma cell-kill for 290 MeV/n carbon ions versus 70 MeV protons in vitro. Radiation Oncology 8: 91, 2013

Comparison of carbon ions versus protons. Cancer Journal 15(4): 325-332, 2010

Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. Experimental Biology and Medicine 242(10): 1015-1024, 2017

Particle therapy using carbon ions or protons as a definitive therapy for patients with primary sacral chordoma. British Journal of Radiology 87(1033): 20130512, 2014

Comparison of the effectiveness of radiotherapy with photons, protons and carbon-ions for non-small cell lung cancer: a meta-analysis. RadioTherapy and Oncology 95(1): 32-40, 2010

Comparison of cellular lethality in DNA repair-proficient or -deficient cell lines resulting from exposure to 70 MeV/n protons or 290 MeV/n carbon ions. Oncology Reports 28(5): 1591-1596, 2012

Direct comparison of biologically optimized spread-out bragg peaks for protons and carbon ions. International Journal of Radiation Oncology Biology Physics 70(1): 262-266, 2008

Measurements of mean energy expended in a gas ion pair formed for protons helium 4 ions and carbon 12 ions in tissue equivalent gas. Radiation Research 76(2): 225-240, 1978

In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs. Radiation Oncology 6: 116, 2011

Genetic changes in progeny of bystander human fibroblasts after microbeam irradiation with X-rays, protons or carbon ions: the relevance to cancer risk. International Journal of Radiation Biology 91(1): 62-70, 2015

Effects of iron ions, protons and X rays on human lens cell differentiation. Radiation Research 164(4 Pt 2): 531-539, 2005

Cell kill by megavoltage protons with high LET. Physics in Medicine and Biology 61(14): 5183-5197, 2016

Molecular characterization of chordoma xenografts generated from a novel primary chordoma cell source and two chordoma cell lines. Journal of Neurosurgery. Spine 21(3): 386-393, 2014

Measuring the spectrum of mutation induced by nitrogen ions and protons in the human-hamster hybrid cell line A(L)C. Radiation Research 153(6): 743-751, 2000

Comparing the use of protons and carbon ions for treatment. Cancer Journal 20(6): 433-439, 2015