+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Effect of 6-BA on the Plant Regeneration via Organogenesis from Cotyledonary Node of Cowpea Vigna unguiculata L Walp

Effect of 6-BA on the Plant Regeneration via Organogenesis from Cotyledonary Node of Cowpea Vigna unguiculata L Walp

Journal of Agricultural Science 5(5)

The present study compares effects of different concentrations of 6-BA on regeneration from cotyledonary node explants of cowpea (Vigna unguiculata L. Walp). The seeds were inoculated on MSB5 medium [Murashige and Skoog (1962) salts and Gamborg B5 vitamins (1968)] containing different concentrations (0, 1, 2, 3, 4, 5 mg/L) of 6-BA for 4 days. The cotyledonary node explants with one cotyledon excised from 4-day-old seedlings, placed in vitro on MSB5 medium containing 6-BA at different dose (0, 0.5, 1.0, 1.5 mg/L) for shoot induction and elongation. Best response in terms of shoot number and shoot length were obtained with explants derived from seedling preconditioning with 3mg/L 6-BA followed by the induction and elongation stage pretreated with 0.5mg/L 6-BA. The elongated shoots were rooted on MSB5 medium without hormone.

(PDF emailed within 0-6 h: $19.90)

Accession: 036852432

Download citation: RISBibTeXText

DOI: 10.5539/jas.v5n5p1

Related references

In vitro plant regeneration via organogenesis of cowpea [Vigna unguiculata (L.) Walp.. Plant Cell Reports 17(2): 89-95, 2019

In vitro regeneration of Vigna unguiculata (L.) Walp. cv. Blackeye cowpea via shoot organogenesis. Plant Cell Tissue and Organ Culture 87(2): 121-125, 2006

In vitro plant regeneration of cowpea (Vigna unguiculata (L.) Walp.) using distal half of cotyledon. Journal of Phytological Research 18(1): 71-75, 2005

Effect of different growth regulators on organogenesis and total fresh weight gain in cultured leaf tissues of cowpea (Vigna unguiculata (L.) Walp.). Phytomorphology 41(1-2): 99-107, 1991

High frequency plant regeneration via somatic embryogenesis in cell suspension cultures of cowpea, Vigna unguiculata (L.) Walp. In Vitro Cellular and Developmental Biology Plant 36(6): 475-480, 2000

Organogenesis in anther-derived callus culture of cowpea (Vigna unguiculata (L.) Walp). Current Science 58(5): 257-259, 1989

Development of a rapid, highly efficient system of organogenesis in cowpea Vigna unguiculata (L.) Walp. South African Journal of Botany 75(1): 17-21, 2009

Effect of plant protection chemicals on Bradyrhizobium in cowpea (Vigna unguiculata Walp). Journal of Tropical Agriculture 34(2): 125-128, 1996

Effect of plant density and spatial arrangement on seed yield of cowpea (Vigna unguiculata (L.) Walp.). Journal of the American Society for Horticultural Science 100(5): 467-470, 1975

Effect of four plant extracts on the infestation of cowpea (Vigna unguiculata (L.) Walp) by Megalurothrips sjostedti (Trybom) and Mylabris pustulata (Thunberg). Global Journal of Pure and Applied Sciences 12(1): 19-21, 2006

Infestation of cowpea ( Vigna unguiculata (L.) Walp.) grain by Sitophilus zeamais (Coleoptera: Curculionidae) and the effect of plant materials on its development. Archives Of Phytopathology And Plant Protection 41(7): 542-544, 2008

Suppression of the cowpea bruchid (Callosobruchus maculatus (F.)) infesting stored cowpea (Vigna unguiculata (L.) Walp.) seeds with some edible plant product powders. Pakistan Journal of Biological Sciences 9(8): 1454-1459, 2006

Drought tolerance of cowpea. 1. Studies on water absorption ability of cowpea (Vigna unguiculata (L.) Walp. var. unguiculata). Japanese Journal of Tropical Agriculture 36(1): 37-44, 1992

Influence of method of land preparation on weed regeneration in cowpea (Vigna unguiculata L. Walp.). Soil and Tillage Research 31(4): 375-383, 1994

Effect of plant density, nitrogen an phosphorus levels on growth and seed yield of cowpea (Vigna unguiculata L. Walp) cv. Pusa Barsati. Orissa Journal of Horticulture 24(1/2): 26-32, 1996