Effect of 6-BA on the Plant Regeneration via Organogenesis from Cotyledonary Node of Cowpea Vigna unguiculata L Walp
Tie, Manman; Luo, Qian; Zhu, Yanxia; Li, Huanxiu
Journal of Agricultural Science 5(5)
ISSN/ISBN: 0021-8596 DOI: 10.5539/jas.v5n5p1
The present study compares effects of different concentrations of 6-BA on regeneration from cotyledonary node explants of cowpea (Vigna unguiculata L. Walp). The seeds were inoculated on MSB5 medium [Murashige and Skoog (1962) salts and Gamborg B5 vitamins (1968)] containing different concentrations (0, 1, 2, 3, 4, 5 mg/L) of 6-BA for 4 days. The cotyledonary node explants with one cotyledon excised from 4-day-old seedlings, placed in vitro on MSB5 medium containing 6-BA at different dose (0, 0.5, 1.0, 1.5 mg/L) for shoot induction and elongation. Best response in terms of shoot number and shoot length were obtained with explants derived from seedling preconditioning with 3mg/L 6-BA followed by the induction and elongation stage pretreated with 0.5mg/L 6-BA. The elongated shoots were rooted on MSB5 medium without hormone.