Section 37
Chapter 36,867

Genetic linkage maps of centipedegrass Eremochloa ophiuroides Munro Hack based on sequence-related amplified polymorphism and expressed sequence tag-simple sequence repeat markers

Zheng, Yiqi; Guo, Hailin; Zang, Guozhang; Liu, Jianxiu

Scientia Horticulturae 156: 86-92


ISSN/ISBN: 0304-4238
DOI: 10.1016/j.scienta.2013.03.030
Accession: 036866143

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Centipedegrass [Eremochloa ophiuroides (Munro) Hack] is a warm-season turfgrass native to the southeast of China. In this study, the first genetic linkage maps of centipedegrass were constructed using PCR-based markers based on a segregation population consisting of 89 F1 progeny derived from a cross between two ecotypes (E102 and E092(1)). A combination of sequence-related amplified polymorphisms (SRAPs) and expressed sequence tag-simple sequence repeats (EST-SSRs) from wheat was used for map construction. Eighty nine loci, including 85 SRAP and 4 EST-SSR loci, in the female (E102) linkage map were placed in five major and five minor (two triplet and three doublets) linkage maps, covering 623.6cM with an average map distance of 7.1cM between adjacent markers. In the male (E092(1)) linkage map, 71 loci including 71 SRAP and 0 EST-SSR loci were assigned to seven major and five triplets linkage maps, with genome coverage of 673.1cM, and a mean inter-marker separation of 9.6cM. The information presented in this study establishes a foundation for extending genetic mapping in this species, serves as a framework for mapping quantitative trait loci, and provides basic information for future molecular breeding.

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