+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Suppression of mRNAs for lipoxygenase LOX, allene oxide synthase AOS, allene oxide cyclase em class=a-p



Suppression of mRNAs for lipoxygenase LOX, allene oxide synthase AOS, allene oxide cyclase em class=a-p







Using a recently developed model pathosystem involving Medicago truncatula and Mycosphaerella pinodes, causal agent of Mycosphaerella blight on pea to understand host molecular response to a fungal suppressor, we applied the suppressor to leaves of M. truncatula and identified 151 nonredundant cDNA fragments as newly expressed genes. These included genes encoding lipoxygenase (LOX) and enoyl-CoA hydratase, which are presumably involved in jasmonic acid (JA) synthesis. Potential genes encoding plastidic enzymes, including allene oxide synthase (AOS) and allene oxide cyclase (AOC), and other peroxisomal enzymes involved in ?-oxidation were predicted from the Medicago Gene Index EST database and tested for altered expression by semiquantitative RT-PCR. The coordinated expression of genes encoding both plastidic and peroxisomal enzymes showed that the suppressor likely conditions certain cellular process(es) through the JA synthesis in M. truncatula. To explore the role of JA or JA-regulated cellular process(es) in conditioning susceptibility, we used an Apple latent spherical virus (ALSV)-based virus-induced gene silencing (VIGS) technology to silence pea genes including LOX, AOS, AOC and 12-oxo-phytodienoic acid reductase (OPR). In LOX-, AOS-, AOC- or OPR-silenced pea plants, disease development induced by M. pinodes was remarkably reduced. Similarly, silencing of mRNA for LOX, AOS, AOC or OPR reduced the sensitivity to a phytotoxin, coronatine, which is believed to act through a JA-dependent process. On the basis of these results, it is conceivable that M. pinodes has evolved a strategy to condition susceptibility by manipulating the physiology of host cells, in particular JA-regulated cellular process(es), to promote disease development in pea.

(PDF emailed within 1 workday: $29.90)

Accession: 036911229

Download citation: RISBibTeXText


Related references

Suppression of mRNAs forlipoxygenase(LOX),allene oxide synthase(AOS),allene oxide cyclase(AOC) and12-oxo-phytodienoic acid reductase(OPR) in pea reduces sensitivity to the phytotoxin coronatine and disease development byMycosphaerella. Journal of General Plant Pathology 79(5): 321-334, 2013

Specific adduction of plant lipid transfer protein by an allene oxide generated by 9-lipoxygenase and allene oxide synthase. Journal of Biological Chemistry 281(51): 38981-8, 2006

Allene Oxide Synthase and Allene Oxide Cyclase, Enzymes of the Jasmonic Acid Pathway, Localized in Glycine max Tissues. Plant Physiology 108(1): 199-202, 1995

Allene oxide synthase, allene oxide cyclase and jasmonic acid levels in Lotus japonicus nodules. Plos One 13(1): E0190884, 2018

Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens. Journal of General Plant Pathology 69(6): 351-357, 2003

Cloning of novel rice allene oxide cyclase mRNA expression and comparative analysis with allene oxide synthase gene provides insight into the transcriptional regulation of octadecanoid pathway biosynthetic genes in rice. Plant Science (Oxford) 164(6): 979-992, 2003

Cloning of novel rice allene oxide cyclase (OsAOC): mRNA expression and comparative analysis with allene oxide synthase (OsAOS) gene provides insight into the transcriptional regulation of octadecanoid pathway biosynthetic genes in rice. Plant Science 164(6): 979-992, 2003

Replacement of two amino acids of 9R-dioxygenase-allene oxide synthase of Aspergillus niger inverts the chirality of the hydroperoxide and the allene oxide. Biochimica et Biophysica Acta 1861(2): 108-118, 2016

Formation of epoxyalcohols by a purified allene oxide synthase. Implications for the mechanism of allene oxide synthesis. Journal of Biological Chemistry 268(9): 6293-6298, 1993

On the relationship of coral allene oxide synthase to catalase. A single active site mutation that induces catalase activity in coral allene oxide synthase. Journal of Biological Chemistry 281(18): 12610-7, 2006

Formation of ketols from linolenic acid 13-hydroperoxide via allene oxide. Evidence for two distinct mechanisms of allene oxide hydrolysis. Biochimica et Biophysica Acta 1086(3): 317-325, 1991

Lipoxygenase-allene oxide synthase pathway in octocoral thermal stress response. Coral Reefs 34(1): 143-154, 2015

Role of radical formation at tyrosine 193 in the allene oxide synthase domain of a lipoxygenase-AOS fusion protein from coral. Biochemistry 42(22): 6871-6880, 2003

Purification and catalytic activities of the two domains of the allene oxide synthase-lipoxygenase fusion protein of the coral Plexaura homomalla. Journal of Biological Chemistry 274(47): 33764-33770, 1999

Molecular cloning, functional expression, and tissue distribution of a potato sprout allene oxide synthase involved in a 9-lipoxygenase pathway. Bioscience, Biotechnology, and Biochemistry 70(9): 2160-2168, 2006