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Electrostatic map of T7 DNA Comparative analysis of functional and electrostatic properties of T7 RNA polymerase specific promoters



Electrostatic map of T7 DNA Comparative analysis of functional and electrostatic properties of T7 RNA polymerase specific promoters







The entire T7 bacteriophage genome contains 39937 base pairs (Database NCBIRefSeq N1001604). Here, electrostatic potential distribution around doublehelical T7 DNA was calculated by Coulomb method using the computer program ofSorokin A.A. Electrostatic profiles of 17 promoters recognized by T7 phagespecific RNA polymerase were analyzed. It was shown that electrostatic profilesof all T7 RNA polymerase specific promoters can be characterized by distinctivemotifs which are specific for each promoter class. Comparative analysis ofelectrostatic profiles of native T7 promoters of different classes demonstratesthat T7 RNA polymerase can differentiate them due to their electrostaticfeatures.

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Accession: 036949470

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