+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Analysis of polyhydroxyalkanoate (PHA) synthase gene and PHA-producing bacteria in activated sludge that produces PHA containing 3-hydroxydodecanoate

Analysis of polyhydroxyalkanoate (PHA) synthase gene and PHA-producing bacteria in activated sludge that produces PHA containing 3-hydroxydodecanoate

Fems Microbiology Letters 346(1): 56-64

ISSN/ISBN: 0378-1097

PMID: 23800349

DOI: 10.1111/1574-6968.12201

Activated sludge is an alternative to pure cultures for polyhydroxyalkanoate (PHA) production due to the presence of many PHA-producing bacteria in activated sludge community. In this study, activated sludge was submitted to aerobic dynamic feeding in a sequencing batch reactor. During domestication, the changes of bacterial community structure were observed by terminal restriction fragment length polymorphism analysis. Furthermore, some potential PHA-producing bacteria, such as Thauera, Acinetobacter and Pseudomonas, were identified by denaturing gradient gel electrophoresis analysis. The constructed PHA synthase gene library was analyzed by DNA sequencing. Of the 80 phaC genes obtained, 76 belonged to the Class I PHA synthase, and four to the Class II PHA synthase. Gas chromatography-mass spectrometry analysis showed that PHA produced by activated sludge was composed of three types of monomers: 3-hydroxybutyrate, 3-hydroxyvalerate and 3-hydroxydodecanoate (3HDD). This is the first report of production of medium-chain-length PHAs (PHAMCL ) containing 3HDD by activated sludge. Further studies suggested that a Pseudomonas strain may play an important role in the production of PHAMCL containing 3HDD. Moreover, a Class II PHA synthase was found to have a correlation with the production of 3HDD-containing PHAMCL .

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036951642

Download citation: RISBibTeXText

Related references

Analysis of polyhydroxyalkanoate (PHA) synthase gene in activated sludge that produces PHA containing 3-hydroxy-2-methylvalerate. Biotechnology and Bioengineering 96(5): 871-880, 2007

Analysis of Medium-Chain-Length Polyhydroxyalkanoate-Producing Bacteria in Activated Sludge Samples Enriched by Aerobic Periodic Feeding. Microbial Ecology 75(3): 720-728, 2018

Increased synthesis of poly(3-hydroxydodecanoate) by random mutagenesis of polyhydroxyalkanoate synthase. Applied Microbiology and Biotechnology 102(18): 7927-7934, 2018

Characterization of polyhydroxyalkanoate-producing bacteria isolated from sludge of commercial pig farms for producing methyl esters. Water Science and Technology: a Journal of the International Association on Water Pollution Research 68(10): 2171-2177, 2013

Isolation and characterization of polyhydroxyalkanoate-accumulating bacteria from activated sludge. Abstracts of the General Meeting of the American Society for Microbiology 95: 444, 1995

Polyhydroxyalkanoate production potential of heterotrophic bacteria in activated sludge. Journal of Bioscience and Bioengineering 121(1): 47-51, 2016

Isolation, characterization and identification of polyhydroxyalkanoate-accumulating bacteria from activated sludge. Journal of Bioscience and Bioengineering 90(5): 494-500, 2000

The isolation and microbial community analysis of hydrogen producing bacteria from activated sludge. Journal of Applied Microbiology 103(5): 1415-1423, 2007

Filamentous bacteria in activated sludge (bulking sludge). I. Occurrence of filamentous bacteria in a sewage treatment plant working with the activated sludge process (plug flow system). Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene, Serie B, Umwelthygiene, Krankenhaushygiene, Arbeitshygiene, praventive Medizin 177(5): 436-450, 1983

Polyhydroxyalkanoate production by a novel bacterium Massilia sp. UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene. Journal of Bioscience and Bioengineering 118(5): 514-519, 2014

A marine bacterium accumulates polyhydroxyalkanoate consisting of mainly 3-hydroxydodecanoate and 3-hydroxydecanoate. World Journal of Microbiology and Biotechnology 26(6): 1149-1153, 2010

Isolation of floc-producing bacteria from activated sludge. Applied Microbiology 1(5): 259-261, 1953

Effects of sludge retention time, carbon and initial biomass concentrations on selection process: From activated sludge to polyhydroxyalkanoate accumulating cultures. Journal of Environmental Sciences 52: 76-84, 2017

Fundamental approach to the activated sludge process I Floc-producing bacteria. Sewage and Industrial Wastes 24(2): 117-123, 1952

A novel microfluidic system enables visualization and analysis of antibiotic resistance gene transfer to activated sludge bacteria in biofilm. Science of the Total Environment 642: 582-590, 2018