+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Substantial within-animal diversity of Salmonella isolates from lymph nodes, feces, and hides of cattle at slaughter

Substantial within-animal diversity of Salmonella isolates from lymph nodes, feces, and hides of cattle at slaughter

Applied and Environmental Microbiology 79(15): 4744-4750

Lymph nodes (mandibular, mesenteric, mediastinal, and subiliac; n = 68) and fecal (n = 68) and hide (n = 35) samples were collected from beef carcasses harvested in an abattoir in Mexico. Samples were analyzed for Salmonella, and presumptive colonies were subjected to latex agglutination. Of the isolates recovered, a subset of 91 was characterized by serotyping, pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility phenotyping. Salmonella was isolated from 100% (hide), 94.1% (feces), 91.2% (mesenteric), 76.5% (subiliac), 55.9% (mandibular), and 7.4% (mediastinal) of samples. From the 87 typeable isolates, eight Salmonella enterica serotypes, including Kentucky (32.2%), Anatum (29.9%), Reading (17.2%), Meleagridis (12.6%), Cerro (4.6%), Muenster (1.1%), Give (1.1%), and Mbandaka (1.1%), were identified. S. Meleagridis was more likely (P = 0.03) to be recovered from lymph nodes than from feces or hides, whereas S. Kentucky was more likely (P = 0.02) to be recovered from feces and hides than from lymph nodes. The majority (59.3%) of the Salmonella isolates were pansusceptible; however, multidrug resistance was observed in 13.2% of isolates. Typing by PFGE revealed that Salmonella strains generally clustered by serotype, but some serotypes (Anatum, Kentucky, Meleagridis, and Reading) were comprised of multiple PFGE subtypes. Indistinguishable PFGE subtypes and, therefore, serotypes were isolated from multiple sample types, and multiple PFGE subtypes were commonly observed within an animal. Given the overrepresentation of some serotypes within lymph nodes, we hypothesize that certain Salmonella strains may be better at entering the bovine host than other Salmonella strains or that some may be better adapted for survival within lymph nodes. Our data provide insight into the ecology of Salmonella within cohorts of cattle and offer direction for intervention opportunities.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 036961881

Download citation: RISBibTeXText

PMID: 23793628

DOI: 10.1128/aem.01020-13

Related references

Antimicrobial drug resistance of Salmonella and Escherichia coli isolates from cattle feces, hides, and carcasses. Journal of Food Protection 70(3): 551-556, 2007

Antimicrobial Drug Resistance of Salmonella and Escherichia coli Isolates from Cattle Feces, Hides, and Carcasses. Journal of Food Protection 70(3): 551-556, 2007

Presence of Salmonella in the mesenteric lymph nodes of slaughter cattle. Mikrobiologija 20(2): 129-131, 1983

Salmonella in Peripheral Lymph Nodes of Healthy Cattle at Slaughter. Frontiers in Microbiology 8: 2214, 2017

Isolation of Salmonella from mesenteric lymph nodes of healthy cattle at slaughter. Research in Veterinary Science 28(2): 238-241, 1980

Salmonella on feces, hides and carcasses in beef slaughter facilities in Venezuela. International Journal of Food Microbiology 166(2): 226-230, 2013

Assessment of Salmonella Prevalence in Lymph Nodes of U.S. and Mexican Cattle Presented for Slaughter in Texas. Journal of Food Protection 2019: 310-315, 2019

Salmonella sp. in small intestine mesenteric lymph nodes hepatic lymph nodes and feces of slaughtered pigs. Medycyna Weterynaryjna 43(8): 465-468, 1987

Reliability of an ordinal rating system for assessing the amount of mud and feces (tag) on cattle hides at slaughter. Journal of Food Protection 62(5): 520-525, 1999

Characterization of O157:H7 and other Escherichia coli isolates recovered from cattle hides, feces, and carcasses. Journal of Food Protection 67(5): 993-998, 2004

Reduction of Escherichia coli O157 and Salmonella in Feces and on Hides of Feedlot Cattle Using Various Doses of a Direct-Fed Microbial. Journal of Food Protection 70(10): 2386-2391, 2007

Reduction of Escherichia coli O157 and Salmonella in feces and on hides of feedlot cattle using various doses of a direct-fed microbial. Journal of Food Protection 70(10): 2386-2391, 2007

Salmonella studies in the Philippines. IV. The isolation of Salmonella from the mesenteric lymph nodes of apparently healthy slaughter swine at the Manila abattoir. Philipp. J. vet. Med, 4: 120-128, 1967

Salmonella fecal shedding in pigs from birth to market and its association with the presence of Salmonella in palatine tonsils and submandibular lymph nodes at slaughter. Canadian Journal of Veterinary Research 82(4): 249-255, 2018

Genetic diversity and antimicrobial resistance among isolates of Escherichia coli O157: H7 from feces and hides of super-shedders and low-shedding pen-mates in two commercial beef feedlots. Bmc Veterinary Research 8: 178, 2013