EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Microspore embryogenesis in wheat: new marker genes for early, middle and late stages of embryo development






Plant Reproduction 26(3): 287-296

Microspore embryogenesis in wheat: new marker genes for early, middle and late stages of embryo development

Microspore embryogenesis involves reprogramming of the pollen immature cell towards embryogenesis. We have identified and characterized a collection of 14 genes induced along different morphological phases of microspore-derived embryo development in wheat (Triticum aestivum L.) anther culture. SERKs and FLAs genes previously associated with somatic embryogenesis and reproductive tissues, respectively, were also included in this analysis. Genes involved in signalling mechanisms such as TaTPD1-like and TAA1b, and two glutathione S-transferase (GSTF2 and GSTA2) were induced when microspores had acquired a star-like morphology or had undergone the first divisions. Genes associated with control of plant development and stress response (TaNF-YA, TaAGL14, TaFLA26, CHI3, XIP-R; Tad1 and WALI6) were activated before exine rupture. When the multicellular structures have been released from the exine, TaEXPB4, TaAGP31-like and an unknown embryo-specific gene TaME1 were induced. Comparison of gene expression, between two wheat cultivars with different response to anther culture, showed that the profile of genes activated before exine rupture was shifted to earlier stages in the low responding cultivar. This collection of genes constitutes a value resource for study mechanism of intra-embryo communication, early pattern formation, cell wall modification and embryo differentiation.


Accession: 036970646

PMID: 23839308

DOI: 10.1007/s00497-013-0225-8



Related references

Magnard, J.L.; L.D.unff, E.; Domenech, J.; Rogowsky, P.M.; Testillano, P.S.; Rougier, M.; Risueño, M.C.; Vergne, P.; Dumas, C., 2001: Genes normally expressed in the endosperm are expressed at early stages of microspore embryogenesis in maize. Reproduction in flowering plants is characterized by double fertilization and the resulting formation of both the zygotic embryo and the associated endosperm. In many species it is possible to experimentally deviate pollen development towards an e...

Muñoz-Amatriaín, M.; Svensson, J.T.; Castillo, A.M.; Close, T.J.; Vallés, M.P., 2009: Microspore embryogenesis: assignment of genes to embryo formation and green vs. albino plant production. Plant microspores can be reprogrammed from their normal pollen development to an embryogenic route in a process termed microspore embryogenesis or androgenesis. Stress treatment has a critical role in this process, inducing the dedifferentiation o...

Vrinten, P.L.; Nakamura, T.; Kasha, K.J., 1999: Characterization of cDNAs expressed in the early stages of microspore embryogenesis in barley L. To gain insight into the molecular events occurring in the very early stages of barley microspore embryogenesis, cDNA clones corresponding to genes differentially expressed during the early stages of microspore culture were isolated and characteri...

Vrinten, P.L.; Nakamura, T.; Kasha, K.J., 1999: Characterization of cDNAs expressed in the early stages of microspore embryogenesis in barley (Hordeum vulgare) L. To gain insight into the molecular events occurring in the very early stages of barley microspore embryogenesis, cDNA clones corresponding to genes differentially expressed during the early stages of microspore culture were isolated and characteri...

Dimov, J.; Zivkova, I., 1967: Early stages of embryogenesis and embryo development in Bulgarian White sows crossed with Landrace boars. A total of 76 purebred Bulgarian Improved White and crossbreds from Landrace x Bulgarian Improved White was used. 72-97 hrs. after the onset of oestrus, the number of ova represented 95.88 and 95.04% of the corporea luta for crossbred and purebred...

Richard, O.; Duittoz, A.H.; Hevor, T.K., 1998: Early, middle, and late stages of neural cells from ovine embryo in primary cultures. The utilization of neural cells in culture has importantly increased the knowledge of the nervous system biology. In most studies, the investigations are performed on biological materials coming from common laboratory animals and the extrapolation...

Alexandrov, W.G.; Alexandrova, O.G., 1939: The early stages of development of the wheat endosperm and embryo. The development of the wheat embryo and endosperm are described and illustrated in some detail. Slight differences were observed between varieties, e.g., as between early and late forms. Environment was found also to exert an influence.

González-Melendi, P.; Ramírez, C.; Testillano, P.S.; Kumlehn, J.; Risueño, Mía.Carmen., 2005: Three dimensional confocal and electron microscopy imaging define the dynamics and mechanisms of diploidisation at early stages of barley microspore-derived embryogenesis. In order to determine the timing and mechanisms of the spontaneous diploidisation throughout microspore-derived embryogenesis in barley, we have estimated the ploidy level of individual nuclei within young pro-embryos, from the first androgenetic...

Solís, Mía-Teresa.; El-Tantawy, A-Abdalla.; Cano, V.; Risueño, Mía.C.; Testillano, P.S., 2015: 5-azacytidine promotes microspore embryogenesis initiation by decreasing global DNA methylation, but prevents subsequent embryo development in rapeseed and barley. Microspores are reprogrammed by stress in vitro toward embryogenesis. This process is an important tool in breeding to obtain double-haploid plants. DNA methylation is a major epigenetic modification that changes in differentiation and proliferati...

Kim, G.Beum.; Lee, R.F., 2004: Effects of genotoxic compounds on DNA and development of early and late grass shrimp embryo stages. Early and late developmental stages of grass shrimp embryos were exposed to different concentrations of two genotoxicants, 2-methyl-1,4-naphthoquinone (MNQ) and 4-nitroquinoline-N-oxide (NQO). DNA strand breaks were assessed by the comet assay whi...