+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Quorum sensing modulates transcription of cpsQ-mfpABC and mfpABC in Vibrio parahaemolyticus



Quorum sensing modulates transcription of cpsQ-mfpABC and mfpABC in Vibrio parahaemolyticus



International Journal of Food Microbiology 166(3): 458-463



Vibrio parahaemolyticus AphA and OpaR are the two master regulators of quorum sensing (QS) that are abundantly produced and operate at low cell density (LCD) and high cell density (HCD), respectively, with an outcome of reciprocally gradient production of these two proteins with transition between LCD and HCD. The cpsQ-mfpABC gene cluster is transcribed as two operons cpsQ-mfpABC and mfpABC in V. parahaemolyticus. MfpABC is a putative membrane fusion transporter that contributes to biofilm development. CpsQ is a c-di-GMP-binding regulator that activates the expression of capsular polysaccharide genes and mfpABC and, thus, induces biofilm development. As shown in this study, OpaR and AphA bind to the promoter region of mfpABC to enhance and repress its transcription, respectively. In contrast, the positive and negative regulation of cpsQ-mfpABC by AphA and OpaR, respectively, achieves probably through acting of AphA or OpaR on additional unknown regulator(s) of cpsQ-mfpABC. The transcriptional levels of cpsQ-mfpABC and mfpABC enhance gradually with transition from LCD to HCD due to the above reciprocal regulatory action of OpaR and AphA. Data presented here present a novel paradigm of combined action of the two master QS regulators in controlling expression of the QS regulon members.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036980842

Download citation: RISBibTeXText

PMID: 24036587

DOI: 10.1016/j.ijfoodmicro.2013.07.008


Related references

Quorum sensing-disrupting brominated furanones protect the gnotobiotic brine shrimp Artemia franciscana from pathogenic Vibrio harveyi, Vibrio campbellii, and Vibrio parahaemolyticus isolates. Applied and Environmental Microbiology 72(9): 6419-6423, 2006

Quorum sensing regulates type III secretion in Vibrio harveyi and Vibrio parahaemolyticus. Journal of Bacteriology 186(12): 3794-3805, 2004

Quorum sensing and silencing in Vibrio parahaemolyticus. Journal of Bacteriology 193(16): 4224-4237, 2011

Identification of a quorum sensing system in Vibrio parahaemolyticus. Abstracts of the General Meeting of the American Society for Microbiology 103: K-093, 2003

Bis-(3'-5')-cyclic dimeric GMP-linked quorum sensing controls swarming in Vibrio parahaemolyticus. Proceedings of the National Academy of Sciences of the United States of America 108(44): 18079-18084, 2012

The Master Quorum-Sensing Regulator OpaR is Activated Indirectly by H-NS in Vibrio parahaemolyticus. Current Microbiology 73(1): 71-76, 2017

Cell density- and quorum sensing-dependent expression of type VI secretion system 2 in Vibrio parahaemolyticus. Plos One 8(8): E73363, 2015

Molecular characterization of direct target genes and cis-acting consensus recognized by quorum-sensing regulator AphA in Vibrio parahaemolyticus. Plos One 7(9): E44210, 2013

Type VI secretion modulates quorum sensing and stress response in Vibrio anguillarum. Environmental Microbiology 11(12): 3018-3028, 2010

Transcriptional regulation of opaR, qrr2-4 and aphA by the master quorum-sensing regulator OpaR in Vibrio parahaemolyticus. Plos One 7(4): E34622, 2012

Stereochemical diversity of AI-2 analogs modulates quorum sensing in Vibrio harveyi and Escherichia coli. Bioorganic and Medicinal Chemistry 20(1): 249-256, 2012

Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi. Bmc Microbiology 15: 159, 2016

The VarS/VarA two-component system modulates the activity of the Vibrio cholerae quorum-sensing transcriptional regulator HapR. Microbiology 157(Pt 6): 1620-1628, 2011

The cyclic AMP receptor protein modulates quorum sensing, motility and multiple genes that affect intestinal colonization in Vibrio cholerae. Microbiology 153(Pt 9): 2964-2975, 2007