+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of New Delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in Escherichia coli isolated from mastitic milk samples



Detection of New Delhi metallo-beta-lactamase and extended-spectrum beta-lactamase genes in Escherichia coli isolated from mastitic milk samples



Transboundary and Emerging Diseases 60(5): 385-389



In this study, eight Escherichia coli isolates were obtained from milk samples of dairy cattle suffering from clinical/subclinical mastitis. Isolates were characterized for antimicrobial resistance traits and virulence genes. Results revealed that one isolate was harbouring New Delhi metallo-beta-lactamase gene (blaNDM ). Cloning and sequencing of the PCR amplicon confirmed the identity of the gene (GenBank accession no. KC769583) having 100% homology with blaNDM-5 (GenBank accession no. JN104597.1), and this isolate was susceptible to colistin, chloramphenicol and tetracycline only. Moreover, another isolate carried extended-spectrum beta-lactamase (ESBL) gene - blaCTX-M , and all isolates possessed blaTEM gene. Of the eight isolates, only one isolate was positive for shiga toxin gene (stx2), and none were harbouring stx1 gene. Occurrence of New Delhi metallo-beta-lactamase (blaNDM ) in one E. coli isolate and ESBL genes in other isolates poses a potential threat to human health following possible entry and spread through food chain.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 037031608

Download citation: RISBibTeXText

PMID: 23870003

DOI: 10.1111/tbed.12119


Related references

Extended spectrum beta-lactamase and metallo beta-lactamase production among Escherichia coli and Klebsiella pneumoniae isolated from different clinical samples in a tertiary care hospital in Kathmandu, Nepal. Annals of Clinical Microbiology and Antimicrobials 16(1): 62, 2018

First identification of an Escherichia coli clinical isolate producing both metallo-beta-lactamase VIM-2 and extended-spectrum beta-lactamase IBC-1. Clinical Microbiology and Infection 10(8): 757-760, 2004

Detection, characterization, and antibiogram of extended-spectrum beta-lactamase Escherichia coli isolated from bovine milk samples in West Bengal, India. Veterinary World 11(10): 1423-1427, 2018

Molecular characterization of an Escherichia coli clinical isolate that produces both metallo-beta-lactamase VIM-2 and extended-spectrum beta-lactamase GES-7: identification of the In8 integron carrying the blaVIM-2 gene. Journal of Antimicrobial ChemoTherapy 58(2): 432-433, 2006

Evaluation of beta-lactamase detection reagent with used HMRZ-86 for the detection of extended-spectrum beta-lactamase and metallo-beta-lactamase. Rinsho Biseibutsu Jinsoku Shindan Kenkyukai Shi 15(1-2): 15-20, 2005

Investigation of beta-lactamase genes and clonal relationship among the extended-spectrum beta-lactamase producing nosocomial Escherichia coli isolates. Mikrobiyoloji Bulteni 49(1): 15-25, 2015

Emergence of multidrug-resistant Klebsiella pneumoniae isolates producing VIM-4 metallo-beta-lactamase, CTX-M-15 extended-spectrum beta-lactamase, and CMY-4 AmpC beta-lactamase in a Tunisian university hospital. Antimicrobial Agents and ChemoTherapy 50(12): 4198-4201, 2006

The association between extended spectrum beta-lactamase (ESBL) and ampicillin C (AmpC) beta-lactamase genes with multidrug resistance in Escherichia coli isolates recovered from turkeys in Brazil. British Poultry Science 59(4): 396-401, 2018

First detection of VIM-1 type metallo-beta-lactamase in a multidrug-resistant Klebsiella pneumoniae clinical isolate from Turkey also producing the CTX-M-15 extended-spectrum beta-lactamase. Journal of ChemoTherapy 19(4): 467-468, 2007

Multidrug resistance mediated by co-carriage of extended-spectrum beta-lactamases, AmpC and New Delhi metallo-beta-lactamase-1 genes among carbapenem-resistant Enterobacteriaceae at five Indian medical centres. Indian Journal of Medical Microbiology 34(3): 359-361, 2017

Molecular Characterization of Extended-Spectrum-beta-Lactamase-Producing and Plasmid-Mediated AmpC beta-Lactamase-Producing Escherichia coli Isolated from Stray Dogs in South Korea. 2012

Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin-clavulanate and piperacillin-tazobactam with extended-spectrum beta-lactamase-producing and extended-spectrum beta-lactamase-non-producing Escherichia coli isolates. Clinical Microbiology and Infection 16(2): 132-136, 2010

Extended-spectrum-beta-lactamase- and AmpC-beta-lactamase-producing Escherichia coli in Dutch broilers and broiler farmers. 2013

Industry survey of Great Britain-sourced broilers for extended-spectrum beta-lactamase and Amp C beta-lactamase-producing Escherichia coli. Veterinary Record 178(19): 474, 2017

Detection of bla(CTX-M-15) extended-spectrum beta-lactamase genes in Escherichia coli from hospital patients in Nigeria. International Journal of Antimicrobial Agents 35(2): 206-207, 2010