EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Improved production of laccase by Daedalea flavida: consideration of evolutionary process optimization and batch-fed culture


Improved production of laccase by Daedalea flavida: consideration of evolutionary process optimization and batch-fed culture



Bioprocess and Biosystems Engineering 37(3): 493-503



ISSN/ISBN: 1615-7591

PMID: 23903597

DOI: 10.1007/s00449-013-1014-3

The genetic algorithm was used effectively to find the optimal values of eight process variables for the maximum laccase production by Daedalea flavida in a stationary culture. The algorithm was modified suitably to improve laccase production with 18 parallel experiments in 4 generations. A high enzyme titer of 65 % was achieved after the optimization and compared to the titer obtained before optimization. To study the effect of the surface immobilized growth on the enzyme production, the fungus was grown on three solid carriers. When cultured on polymer composite fibers, polyurethane foam, or steel wool, at least 2.5 times more biomass was produced, compared to the biomass produced in support-free growth. On the contrary, the mycelia grown on solid support produced much less laccase than non-adhering mycelia. Four parallel runs of batch-fed cultures were done, using the cell mass of D. flavida to evaluate the influence of four different volumes of medium exchanged on laccase production. For sustainable production of the enzyme, complete exchange of medium was favorable, where the laccase activity increased continuously in six consecutive cycles, though, 50 % exchange of medium produced the maximum laccase in terms of mean enzyme activity obtained in six cycles.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 037258018

Download citation: RISBibTeXText

Related references

Optimization of laccase fermentation and evaluation of kinetic and thermodynamic parameters of a partially purified laccase produced by Daedalea flavida. Preparative Biochemistry and Biotechnology 45(4): 307-335, 2015

Laccase production by daedalea flavida on different substrates. International Union Of Microbiological Societies 13th International Congress Of Microbiology; Boston, Mass , Usa, Aug 8-13, Xiv+182p American Society For Microbiology: Washington, D C , Usa Paper P143, 1982

Laccase production and wood degradation by a white-rot fungus Daedalea flavida. Enzyme and Microbial Technology 7(8): 405-408, 1985

Study on optimization of culture conditions for improved production of Lentinula edodes laccase. Chemistry and Industry of Forest Products 26(2): 74-78, 2006

Pretreatment of cotton stalks by synergistic interaction of Daedalea flavida and Phlebia radiata in co-culture for improvement in delignification and saccharification. International Biodeterioration and Biodegradation 117: 68-77, 2017

Production of laccase by Coriolus versicolor and its application in decolorization of dyestuffs: (I). Production of laccase by batch and repeated-batch processes. Journal of Environmental Sciences 15(1): 1-4, 2003

Optimization of culture conditions for laccase production by Trametes versicolor and dye decolorization by the crude laccase. Chemistry and Industry of Forest Products 26(3): 41-46, 2006

Optimization of Bioethanol In Silico Production Process in a Fed-Batch Bioreactor Using Non-Linear Model Predictive Control and Evolutionary Computation Techniques. Energies 10(11): 1763, 2017

Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity. Bioprocess and Biosystems Engineering 37(7): 1401-1409, 2014

Improved elastase production by Bacillus sp. EL31410--further optimization and kinetics studies of culture medium for batch fermentation. Journal of Zhejiang University. Science 5(2): 149-156, 2004

Agave tequilana bagasse for methane production in batch and sequencing batch reactors: Acid catalyst effect, batch optimization and stability of the semi-continuous process. Journal of Environmental Management 224: 156-163, 2018

Interfertility study of Daedalea flavida Lev. Current Science 49(1): 33-34, 1980

Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture. International Journal of Biological Macromolecules 108: 999-1009, 2018

Host-range and distribution of Daedalea flavida Lev. Indian J. mycol. Res 2(1-2): 76-77, 1956

Improved batch process performance by evolutionary modelling. I-Cimpro International Conference on Computer Integrated Manufacturing in the Process Industries 36(3): 271-278, 1998